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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Promising effects of silver tungstate microcrystals on fibroblast human cells and three dimensional collagen matrix models: A novel non-cytotoxic material to fight oral disease

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Haro Chavez, Natali Lisette [1] ; de Avila, Erica Dorigatti [1] ; Barbugli, Paula Aboud [1] ; de Oliveira, Regiane Cristina [2] ; de Foggi, Camila Cristina [1] ; Longo, Elson [2] ; Vergani, Carlos Eduardo [1]
Total Authors: 7
[1] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil
[2] Univ Fed Sao Carlos, CDMF, UFSCar, Washington Luis Km 235, POB 676, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 170, p. 505-513, OCT 1 2018.
Web of Science Citations: 3

Silver tungstate (alpha-Ag2WO4) microcrystals have shown encouraging results regarding their antimicrobial activity. However, in addition to the promising outcomes in fighting oral disease, cytotoxic tests are mandatory for screening new materials for biological applications. Here, we developed a better understanding of the effects of microcrystals on the behavior of both human gingival fibroblast (HGF) cells and three-dimensional (3D) collagen matrices. To perform these experiments, the lowest concentration of alpha-Ag2WO4 capable of preventing the visible growth of Candida albicans (C. albicans) planktonic cells was defined as the test concentration, and it ranged from 0.781 (C1) to 7.81 (C2) to 78.1 (C3) mu g/mL. Complete medium and lysis buffer (LB) served as negative (C-) and positive (C+) controls, respectively. The effect of the microcrystal concentration on the morphology, remodeling and proliferation of HGF cells was evaluated by different approaches. Quantitative and qualitative assessments demonstrated that alpha-Ag2WO4 did not affect the mitochondria] enzymatic activity of HGF cells cultured in a monolayer or the cell viability within 3D collagen matrices. These experiments showed that alpha-Ag2WO4 at the C2 concentration did not damage the genomic DNA. The development of new materials is attractive for the possible treatment of diseases and for avoiding indiscriminate prescribing of antibiotics. These findings provide information on the effect of alpha-Ag2WO4 on cell behavior and reveal that these microcrystals are non-cytotoxic against human gingival cells over a sufficient period to measure the hazard potential. (AU)

FAPESP's process: 15/25124-0 - Effect of antimicrobial and cytotoxic properties of ±-Ag2WO4 and ²-Ag2MoO4 as titanium coating materials surfaces
Grantee:Carlos Eduardo Vergani
Support type: Regular Research Grants
FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
Grantee:Elson Longo da Silva
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 15/03654-7 - Antimicrobial activity of bimetallic oxides and of plasma-deposited film on denture base acrylic resin
Grantee:Camila Cristina de Foggi
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 15/03567-7 - Modification of the physicochemical properties with different coating materials of titanium implant abutment surfaces and evaluation of their effects on the development of a polymicrobial community representative of the oral cavity: an in vitro preliminar
Grantee:Érica Dorigatti de Avila
Support type: Scholarships in Brazil - Post-Doctorate