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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation of spilanthol from Acmella oleracea based on Green Chemistry and evaluation of its in vitro anti-inflammatory activity

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de Freitas Blanco, Veronica Santana [1, 2] ; Michalak, Barbara [3] ; Maccari Zelioli, Icaro Augusto [4] ; Santos de Oliveira, Adriana da Silva [5] ; Nova Rodrigues, Marili Villa [5] ; Ferreira, Antonio Gilberto [6] ; Garcia, Vera Lucia [5] ; Cabral, Fernando Antonio [4] ; Kiss, Anna Karolina [3] ; Ferreira Rodrigues, Rodney Alexandre [1, 2]
Total Authors: 10
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Pharmacol Anesthesiol & Therapeut, Piracicaba, SP - Brazil
[2] Univ Estadual Campinas, Dept Chem Nat Prod, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, Campinas, SP - Brazil
[3] Med Univ Warsaw, Dept Pharmacognosy & Mol Basis Phytotherapy, Warsaw - Poland
[4] Univ Estadual Campinas, Dept Food Engn, Food Engn Fac, Lab Extract Appl Thermodynam & Equilibrium, Campinas, SP - Brazil
[5] Univ Estadual Campinas, Dept Organ & Pharmaceut Chem, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, Campinas, SP - Brazil
[6] Univ Fed Sao Carlos, Chem Dept, NMR Lab, Sao Carlos, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: JOURNAL OF SUPERCRITICAL FLUIDS; v. 140, p. 372-379, OCT 2018.
Web of Science Citations: 1

Acmella oleracea (jambu) is a South American plant whose pharmacological properties include antimalarial, anesthetic, and anti-inflammatory activities. However, the compounds contributing to the anti-inflammatory effect have received little attention. In this study, the active compound spilanthol was isolated using a Green Chemistry approach and presented significant anti-inflammatory activity in a lipopolysaccharide-activated human neutrophil model. First, the aerial parts of jambu were extracted and fractionated using supercritical carbon dioxide. The spilanthol-enriched fractions obtained were then subjected to further purification by flash chromatography, yielding spilanthol with 97% purity. Use of enzyme-linked immunosorbent assay (ELISA) revealed a reduction in the release of Interleukin-8 and Tumor Necrosis Factor - alpha by leukocytes exposed to spilanthol. In conclusion, this new approach enabled spilanthol to be obtained at high purity, in a fairly rapid procedure, while the results of the in vitro anti-inflammatory activity study indicated that the compound could be a promising new therapeutic agent. (AU)

FAPESP's process: 14/06461-2 - Extraction of espilantol in the context of green chemistry and its application in the treatment of oral mucositis
Grantee:Verônica Santana de Freitas Blanco
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 15/09644-3 - Non-isothermal separation process using supercritical technology and thermal diffusion
Grantee:Fernando Antonio Cabral
Support Opportunities: Regular Research Grants
FAPESP's process: 14/07225-0 - Obtainment of N-alkilamide (Spilanthol) from aerial parts of jambu, using supercritical technology
Grantee:Ícaro Augusto Maccari Zelioli
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/16186-9 - Development and evaluation of topical formulations for oral mucositis treatment containing Spilanthol
Grantee:Rodney Alexandre Ferreira Rodrigues
Support Opportunities: Regular Research Grants