Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Suppression of Plant Immunity by Fungal Chitinase-like Effectors

Full text
Author(s):
Fiorin, Gabriel Lorencini [1, 2] ; Sanchez-Vallet, Andrea [3, 4] ; de Toledo Thomazella, Daniela Paula [5] ; Vital do Prado, Paula Favoretti [1, 2] ; do Nascimento, Leandro Costa [2, 6] ; de Oliveira Figueira, Antonio Vargas [7] ; Thomma, Bart P. H. J. [3] ; Guimaraes Pereira, Goncalo Amarante [2] ; Pereira Lima Teixeira, Paulo Jose [8]
Total Authors: 9
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Grad Program Genet & Mol Biol, BR-13083970 Campinas - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, Lab Genom & Expressao, BR-13083970 Campinas - Brazil
[3] Wageningen Univ & Res, Lab Phytopathol, Droevendaalsesteeg 1, NL-6708 Wageningen - Netherlands
[4] Swiss Fed Inst Technol, Inst Integrat Biol, Plant Pathol, CH-8092 Zurich - Switzerland
[5] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 - USA
[6] Univ Estadual Campinas, Ctr Nacl Processamento Alto Desempenho, BR-13083970 Campinas - Brazil
[7] Univ Sao Paulo, Ctr Energia Nucl Agr, Lab Melhoramento Plantas, Campus Luiz de Queiroz, BR-13400970 Piracicaba - Brazil
[8] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 - USA
Total Affiliations: 8
Document type: Journal article
Source: Current Biology; v. 28, n. 18, p. 3023+, SEP 24 2018.
Web of Science Citations: 5
Abstract

Crop diseases caused by fungi constitute one of the most important problems in agriculture, posing a serious threat to food security {[}1]. To establish infection, phytopathogens interfere with plant immune responses {[}2, 3]. However, strategies to promote virulence employed by fungal pathogens, especially non-model organisms, remain elusive {[}4], mainly because fungi are more complex and difficult to study when compared to the better-characterized bacterial pathogens. Equally incomplete is our understanding of the birth of microbial virulence effectors. Here, we show that the cacao pathogen Moniliophthora perniciosa evolved an enzymatically inactive chitinase (MpChi) that functions as a putative pathogenicity factor. MpChi is among the most highly expressed fungal genes during the bio-trophic interaction with cacao and encodes a chitinase with mutations that abolish its enzymatic activity. Despite the lack of chitinolytic activity, MpChi retains substrate binding specificity and prevents chitin-triggered immunity by sequestering immunogenic chitin fragments. Remarkably, its sister species M. roreri encodes a second non-orthologous catalytically impaired chitinase with equivalent function. Thus, a class of conserved enzymes independently evolved as putative virulence factors in these fungi. In addition to unveiling a strategy of host immune suppression by fungal pathogens, our results demonstrate that the neo-functionalization of enzymes may be an evolutionary pathway for the rise of new virulence factors in fungi. We anticipate that analogous strategies are likely employed by other pathogens. (AU)

FAPESP's process: 11/23315-1 - Functional characterization of a chitinase-like protein of Moniliophthora perniciosa with a potential role in fungal pathogenicity during the development of the witches' broom disease of cacao.
Grantee:Gabriel Lorencini Fiorin
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/10498-4 - Investigation of strategies of adaptation to the pathogenic life style of fungi from the Moniliophthora genus at various levels of biological organizations: species, biotypes, and geographic lineages
Grantee:Antonio Vargas de Oliveira Figueira
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/06181-0 - Development of a CRISPR/Cas9-based methodology for genome engineering in the fungus Moniliophthora perniciosa, the causative agent of witches' broom disease of cacao
Grantee:Gabriel Lorencini Fiorin
Support type: Scholarships in Brazil - Master
FAPESP's process: 09/50119-9 - Integrated and comparative study of three fungal diseases of cacao: witches' broom, frosty pod rot and brown-rot, aiming at understanding the pathogenic mechanisms for the development of control strategies
Grantee:Gonçalo Amarante Guimarães Pereira
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/09878-9 - Functional characterization of a chitinase-like protein of Moniliophthora perniciosa with a potential role in fungal pathogenicity during the development of the witches' broom disease of cacao
Grantee:Gabriel Lorencini Fiorin
Support type: Scholarships abroad - Research Internship - Scientific Initiation