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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comprehensive high-resolution multiple-reaction monitoring mass spectrometry for targeted eicosanoid assays

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Sorgi, Carlos Arterio [1] ; Ferranti Peti, Ana Paula [1] ; Petta, Tania [1] ; Galvao Meirelles, Alyne Favero [1] ; Fontanari, Caroline [1] ; Beraldo de Moraes, Luiz Alberto [2] ; Faccioli, Lucia Helena [1]
Total Authors: 7
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Av Bandeirantes 3900, BR-14049901 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: SCIENTIFIC DATA; v. 5, AUG 21 2018.
Web of Science Citations: 2

Eicosanoids comprise a class of bioactive lipids derived from a unique group of essential fatty acids that mediate a variety of important physiological functions. Owing to the structural diversity of these lipids, their analysis in biological samples is often a major challenge. Advancements in mass spectrometric have been helpful for the characterization and quantification of these molecular lipid species in complex matrices. However, there are technical limitations to this approach, including low-abundant and/or poorly ionizable lipids. Using high-resolution multiple-reaction monitoring (MRMHR), we were able to develop a targeted bioanalytical method for eicosanoid quantification. For this, we optimized the LC-MS/MS conditions and evaluated several parameters, including linearity, limits of quantification, matrix effects and recovery yields. For validation purposes, we looked at the method's precision and accuracy. A library of high-resolution fragmentation spectra for eicosanoids was developed. Our comprehensive dataset meets benchmark standards for targeted analysis, having been derived using best-practice workflows and rigorous quality assessments. As such, our method has applications for determining complex eicosanoid profiles in the biomedical field. (AU)

FAPESP's process: 14/07125-6 - New functional aspects of eicosanoids
Grantee:Lúcia Helena Faccioli
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/16788-4 - Simultaneous identification and quantification of cytochrome-P450 pathway (CYP450)-derived eicosanoids using high performance liquid chromatography coupled to sequential mass spectrometry (HPLC-MS/MS)
Grantee:Ana Paula Ferranti Peti
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 15/00658-1 - New functional aspects of eicosanoids
Grantee:Lúcia Helena Faccioli
Support Opportunities: Multi-user Equipment Program