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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High-Resolution Genetic Map and QTL Analysis of Growth-Related Traits of Hevea brasiliensis Cultivated Under Suboptimal Temperature and Humidity Conditions

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Author(s):
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Conson, Andre R. O. [1] ; Taniguti, Cristiane H. [2] ; Amadeu, Rodrigo R. [2] ; Andreotti, Isabela A. A. [1] ; de Souza, Livia M. [1] ; dos Santos, Luciano H. B. [1] ; Rosa, Joao R. B. F. [2, 3] ; Mantello, Camila C. [1, 4] ; da Silva, Carla C. [1] ; Scaloppi Junior, Erivaldo Jose [5] ; Ribeiro, Rafael V. [6] ; Le Guen, Vincent [7] ; Garcia, Antonio A. F. [2] ; Goncalves, Paulo de Souza [5] ; de Souza, Anete P. [6, 1]
Total Authors: 15
Affiliation:
[1] Univ Estadual Campinas, Mol Biol & Genet Engn Ctr, Campinas, SP - Brazil
[2] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Genet, Piracicaba - Brazil
[3] FTS Sementes SA, Ctr Res & Dev, Ponta Grossa - Brazil
[4] NIAB, Cambridge - England
[5] Agron Inst IAC, Ctr Rubber Tree & Agroforestry Syst, Votuporanga - Brazil
[6] Univ Estadual Campinas, Dept Plant Biol, Inst Biol, Campinas, SP - Brazil
[7] French Agr Res Ctr Int Dev CIRAD, UMR AGAP, Montpellier - France
Total Affiliations: 7
Document type: Journal article
Source: FRONTIERS IN PLANT SCIENCE; v. 9, AUG 24 2018.
Web of Science Citations: 4
Abstract

Rubber tree (Hevea brasiliensis) cultivation is the main source of natural rubber worldwide and has been extended to areas with suboptimal climates and lengthy drought periods; this transition affects growth and latex production. High-density genetic maps with reliable markers support precise mapping of quantitative trait loci (QTL), which can help reveal the complex genome of the species, provide tools to enhance molecular breeding, and shorten the breeding cycle. In this study, QTL mapping of the stem diameter, tree height, and number of whorls was performed for a full-sibling population derived from a GT1 and RRIM701 cross. A total of 225 simple sequence repeats (SSRs) and 186 single-nucleotide polymorphism (SNP) markers were used to construct a base map with 18 linkage groups and to anchor 671 SNPs from genotyping by sequencing (GBS) to produce a very dense linkage map with small intervals between loci. The final map was composed of 1,079 markers, spanned 3,779.7 cM with an average marker density of 3.5 cM, and showed collinearity between markers from previous studies. Significant variation in phenotypic characteristics was found over a 59-month evaluation period with a total of 38 QTLs being identified through a composite interval mapping method. Linkage group 4 showed the greatest number of QTLs (7), with phenotypic explained values varying from 7.67 to 14.07%. Additionally, we estimated segregation patterns, dominance, and additive effects for each QTL. A total of 53 significant effects for stem diameter were observed, and these effects were mostly related to additivity in the GT1 clone. Associating accurate genome assemblies and genetic maps represents a promising strategy for identifying the genetic basis of phenotypic traits in rubber trees. Then, further research can benefit from the QTLs identified herein, providing a better understanding of the key determinant genes associated with growth of Hevea brasiliensis under limiting water conditions. (AU)

FAPESP's process: 14/11807-5 - Use of genotyping-by-sequencing (GBS) for the identification of molecular markers SNP (single nucleotide polymorphism) and construction of the linkage map in Hevea brasiliensis
Grantee:Luciano Henrique Braz dos Santos
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/50491-8 - Evaluation of allelic variability, population structure and linkage desequilibrium in the rubber tree ex situ germoplasm (Hevea brasiliensis)
Grantee:Anete Pereira de Souza
Support type: Regular Research Grants
FAPESP's process: 15/24346-9 - Allelic variation analysis in genes of economic importance among hom(oe)ologous regions of sugarcane (Saccharum spp.) genome
Grantee:Carla Cristina da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/07908-9 - Genetic and genomic analysis in the Hevea genus aiming to contribute to the genetic improvement of Hevea brasiliensis
Grantee:Luciano Henrique Braz dos Santos
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 12/05473-1 - Evaluation of the linkage desequilibrium and population structure in rubber tree ex situ germplasm (Hevea brasiliensis) using SNPs (Single Nucleotide Polymorphism)
Grantee:Livia Moura de Souza
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/18755-0 - Analysis of RNA expression profiling by RNA-seq in rubber tree and identification of the genes involved in the response to cold
Grantee:Camila Campos Mantello
Support type: Scholarships in Brazil - Post-Doctorate