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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Label-free quantitative proteomic analysis reveals muscle contraction and metabolism proteins linked to ultimate pH in bovine skeletal muscle

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Author(s):
Poleti, Mirele Daiana [1, 2] ; Moncau, Cristina Tschorny [1] ; Silva-Vignato, Barbara [1] ; Rosa, Alessandra Fernandes [1] ; Lobo, Adalfredo Rocha [1, 3] ; Cataldi, Thais Regiani [4] ; Negrao, Joao Alberto [1] ; Silva, Saulo L. [5] ; Eler, Joanir Pereira [1] ; de Carvalho Balieiro, Julio Cesar [1, 6]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, FZEA, Coll Anim Sci & Food Engn, Dept Basic Sci, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Sao Paulo, Luiz de Queiroz Coll Agr, ESALQ, BR-13418900 Piracicaba, SP - Brazil
[3] Fed Univ Vales Jequitinhonha & Mucuri, BR-38610000 Unai, MG - Brazil
[4] Univ Sao Paulo, ESALQ, Luiz de Queiroz Coll Agr, Depr Genet, BR-13418900 Piracicaba, SP - Brazil
[5] Univ Sao Paulo, Dept Anim Sci, Coll Anim Sci & Food Engn, FZEA, Pirassununga, SP - Brazil
[6] Univ Sao Paulo, Coll Vet Med & Anim Sci, BR-13635900 Pirassununga, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: MEAT SCIENCE; v. 145, p. 209-219, NOV 2018.
Web of Science Citations: 1
Abstract

The purpose of this research was to investigate the causes and consequences of pHu variations in beef cattle. A group of 176 Nellore beef cattle was evaluated and classified into two different pHu groups: High (>= 6.0, N = 17) and Normal (< 5.8, N = 159). Plasma concentrations of cortisol and adrenocorticotropic hormone, lactate and glycogen muscular content, meat color, shear force and Longissimus thoracis muscle proteomic profile were evaluated and compared between pHu groups. Muscle glycogen content, meat color and shear force statistically differed between pHu groups. Label-free quantitative proteomic analysis revealed ten differentially abundant proteins between pHu groups, involved in metabolic processes and muscle contraction, which also were significantly correlated with pHu. Thirty-six and 31 proteins were exclusively present in Normal and High pHu group, respectively, which were related to TCA cycle, cortisol production, calcium regulation, and antioxidant function. The MYH7, UGP2, H2AFJ and VDAC3 were identified as potential indicators of pHu variations. CALM and NNT appeared to be interesting proteins to understand the metabolic pathways behind pHu. Data are available via ProteomeXchange with identifier PXD009320. (AU)

FAPESP's process: 10/07057-0 - Variation in the mineralocorticoid and glucocorticoid receptors gene and proteomics implications for meat quality in cattle of Nellore breed
Grantee:Mirele Daiana Poleti
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/07078-7 - Variation in the mineralocorticoid and glucocorticoid receptors genes and proteomics implications for meat quality in cattle of Nellore breed
Grantee:Júlio Cesar de Carvalho Balieiro
Support Opportunities: Regular Research Grants