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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prevalence and molecular features of ESBL/pAmpC-producing Enterobacteriaceae in healthy and diseased companion animals in Brazil

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Melo, Luana C. [1, 2] ; Oresco, Cintia [1] ; Leigue, Lucianne [1] ; Netto, Hildebrando M. [3] ; Melville, Priscilla A. [4] ; Benites, Nilson R. [4] ; Saras, Estelle [2] ; Haenni, Marisa [2] ; Lincopan, Nilton [1] ; Madec, Jean-Yves [2]
Total Authors: 10
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil
[2] Univ Claude Bernard Lyon 1, Unite Antibioresistance & Virulence Bacteriennes, ANSES Lab Lyon, Lyon - France
[3] Ctr Zoonosis Control, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Prevent Vet Med & Anim Hlth, Fac Vet Med, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Veterinary Microbiology; v. 221, p. 59-66, JUL 2018.
Web of Science Citations: 4

Extended-spectrum beta-lactamase (ESBL)-and plasmid-mediated AmpC (pAmpC)-carrying Enterobacteriaceae have widely disseminated in human, animal and environmental reservoirs. Pets have been recognized as a source of ESBL/pAmpC worldwide, and are possibly also a source of human contamination. The aim of this study was to document to what extent cats and dogs may act as a driving force in the spread of ESBLs and pAmpCs in Brazil. A total of 113 healthy stray cats and dogs and 74 sick pets were sampled, and extended-spectrum cephalosporinresistant Enterobacteriaceae (ESC-R) were detected in 28/113 (24.8%) and 8/74 (10.8%) tested animals, respectively. Different Enterobacteriaceae isolates (mostly E. coli), a large number of E. coli clones (with ST90, ST457, ST973 and ST2541 being predominant), and several ESBL/pAmpC genes and plasmids were characterized, highlighting the ability of stray and pet cats and dogs to further spread a wide range of ESC-resistance determinants. The ESBL phenotype was due to the bla(CTX-M-2) and bla(CTX-M-8) genes, as found in human epidemiology in Brazil, but bla(CTX.M.9) and bla(CTX-M-15) were also identified. The pAmpC phenotype was systematically due to the presence of the bla(CMY-2) gene, mostly carried by Incl1 ST12 plasmids. Our results showed that pets can be considered a significant reservoir of multidrug-resistant bacteria in Brazil. This is especially true for healthy stray dogs that displayed the highest prevalence (24.8%) of ESBLs/pAmpC resistance determinants, which can then be further spread both to the environment and to other animals or humans by contact. (AU)

FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
Grantee:Nilton Erbet Lincopan Huenuman
Support Opportunities: Regular Research Grants
FAPESP's process: 13/04725-0 - Monitoring of multidrug-resistant Gram-negative bacteria of medical importance (human & veterinary): clinical/environmental impact and development of therapeutic alternatives and technology innovation products
Grantee:Nilton Erbet Lincopan Huenuman
Support Opportunities: Regular Research Grants