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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

NLRP3 inflammasome is involved in the recognition of Paracoccidioides brasiliensis by human dendritic cells and in the induction of Th17 cells

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Author(s):
de Castro, Livia Furquim [1] ; Alegrini Longhi, Larissa Nara [1] ; Paiao, Munir Regini [1] ; Justo-Junior, Amauri da Silva [1] ; de Jesus, Marcelo Bispo [2] ; de Souza Lima Blotta, Maria Heloisa [1] ; Mamoni, Ronei Luciano [1, 3]
Total Authors: 7
Affiliation:
[1] Univ Campinas UNICAMP, Fac Med Sci, Dept Clin Pathol, BR-13083970 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
[3] FMJ, Dept Morphol & Basic Pathol, Jundiai, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Infection; v. 77, n. 2, p. 137-144, AUG 2018.
Web of Science Citations: 2
Abstract

Objectives: To investigate the involvement of NLRP3 in the effector functions of human dendritic cells (DCs) in response to Paracoccidioides brasiliensis yeast cells (Pb) and to evaluate its role in the modulation of the adaptive immune response. Methods: DCs were differentiated from purified peripheral blood monocytes and analyzed in relation to the participation of TLR-2,dectin-1, and Syk in Pb recognition, as well as, the indirect mechanisms (Reactive Oxygen Species production, endosome acidification, or K+ efflux) involved in NLRP3 inflammasome activation after the stimulus with Pb. Additionally, we analyzed the role of NLRP3 in the activation of T cells. Results: Our results demonstrated that the NLRP3 inflammasome activation and cytokines production by DCs are dependent on ROS generation, endosome acidification, and K+ efflux and involve the Pb recognition by dectin-1 and Syk phosphorylation. Our data also demonstrate that the NLRP3 inflammasome is essential for the activation/expansion of Th1/Th17 cells and its inhibition leads to an increased frequency of Th2 and Treg cells. Conclusion: Altogether our data indicated that activation of NLRP3 presents an important role in both the induction of the initial inflammatory response and in the development of the acquired immune response associated with resistance to infection. (C) 2018 The British Infection Association. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 13/03673-6 - Study of the inflamassoma NLRP3 in the inflammatory response induced by the dimorphic fungus Paracoccidioides brasiliensis
Grantee:Lívia Furquim de Castro
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 13/24286-0 - Participation of the inflammasome NLRP3 and cytokines from the IL-1 family in the infammatory response induced by the pathogenic fungi Paracoccidioides brasiliensis and Candida albicans
Grantee:Ronei Luciano Mamoni
Support Opportunities: Regular Research Grants