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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro susceptibilities of Neoscytalidium spp. sequence types to antifungal agents and antimicrobial photodynamic treatment with phenothiazinium photosensitizers

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Tonani, Ludmilla [1, 2] ; Morosini, Natalia Silva [1] ; de Menezes, Henrique Dantas [1] ; Nadaletto Bonifacio da Silva, Maria Emilia [1] ; Wainwright, Mark [3] ; Leite Braga, Gilberto Ubida [1] ; von Zeska Kress, Marcia Regina [1]
Total Authors: 7
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil
[3] Liverpool John Moores Univ, Sch Pharm & Biomol Sci, Liverpool L3 3AF, Merseyside - England
Total Affiliations: 3
Document type: Journal article
Source: FUNGAL BIOLOGY; v. 122, n. 6, SI, p. 436-448, JUN 2018.
Web of Science Citations: 4

Neoscytalidium spp. are ascomycetous fungi consisting of pigmented and hyaline varieties both able to cause skin and nail infection. Their color-based identification is inaccurate and may compromise the outcome of the studies with these fungi. The aim of this study was to genotype 32 isolates morphologically identified as Neoscytalidium dimidiatum or N. dimidiatum var. hyalinum by multilocus sequence typing (MLST), differentiate the two varieties by their sequence types, evaluate their susceptibility to seven commercial antifungal drugs lamphotericin B (AMB), voriconazole (VOR), terbinafine (TER), 5-flucytosine (5FC), ketoconazole (KET), fluconazole (FLU), and caspofungin (CAS)], and also to the antimicrobial photodynamic treatment (APDT) with the phenothiazinium photosensitizers (PS) methylene blue (MB), new methylene blue (NMBN), toluidine blue 0 (TBO) and the pentacyclic derivative 5137. The efficacy of each PS was determined, initially, based on its minimal inhibitory concentration (MIC). Additionally, the APDT effects with each PS on the survival of ungerminated and germinated arthroconidia of both varieties were evaluated. Seven loci of Neoscytalidium spp. were sequenced on MLST revealing eight polymorphic sites and six sequence types (ST). All N. dimidiatum var. hyalinum isolates were clustered in a single ST. AMB, VOR and TER were the most effective antifungal agents against both varieties. The hyaline variety isolates were much less tolerant to the azoles than the isolates of the pigmented variety. APDT with 5137 showed the lowest MIC for all the isolates of both varieties. APDT with all the PS killed both ungerminated and germinated arthroconidia of both varieties reducing the survival up to 5 logs. Isolates of the hyaline variety were also less tolerant to APDT. APDT with the four PS also increased the plasma membrane permeability of arthroconidia of both varieties but only NMBN and S137 caused peroxidation of the membrane lipids. (C) 2017 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/23920-5 - Susceptibility characterization of Scytalidium sp. clinical isolates to antifungi
Grantee:Natalia Silva Morosini
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/17028-2 - Susceptibility characterization of Scytalidium sp. clinical isolates to antifungi and photosensitizers
Grantee:Marcia Regina von Zeska Kress
Support Opportunities: Regular Research Grants
FAPESP's process: 16/11386-5 - Mechanistic study of perception and response to visible light in the entomopathogenic fungus Metarhizium acridum
Grantee:Gilberto Úbida Leite Braga
Support Opportunities: Regular Research Grants