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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese mice

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Author(s):
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de Souza Teixeira, Alexandre Abilio [1] ; Souza, Camila O. [1] ; Biondo, Luana A. [1] ; Silveira, Loreana Sanches [2] ; Lima, Edson A. [1] ; Batatinha, Helena A. [1] ; Araujo, Adriane Pereira [1] ; Alves, Michele Joana [1] ; Hirabara, Sandro Massao [3] ; Curi, Rui [4, 3] ; Rosa Neto, Jose Cesar [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell Biol & Dev, Immunometab Res Grp, Sao Paulo, SP - Brazil
[2] UNESP, Dept Phys Educ, Exercise & Immunometab Res Grp, Presidente Prudente - Brazil
[3] Univ Cruzeiro Sul, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: INFLAMMOPHARMACOLOGY; v. 26, n. 4, p. 1103-1115, AUG 2018.
Web of Science Citations: 4
Abstract

The study aimed to evaluate the metabolic and inflammatory effects of short-term treatments (10 days) with metformin (MET) on the NAFLD caused by a high-fat diet (HFD) in C57BL/6 mice. After the treatment, histological liver slices were obtained, hepatocytes and macrophages were extracted and cultured with phosphate buffered saline, LPS (2.5 A mu g/mL) and MET (1 A mu M) for 24 h. Cytokine levels were determined by ELISA. NAFLD caused by the HFD was partially reduced by MET. The lipid accumulation induced by the HFD was not associated with liver inflammation; however, MET seemed to promote pro-inflammatory effects in liver, since it increased hepatic concentration of IL-1 beta, TNF-alpha, IL-6, MCP-1 and IFN-gamma. Similarly, MET increased the concentration of IL-1 beta, IL-6 in hepatocyte cultures. However, in macrophages culture, MET lowered levels of IL-1 beta, IL-6 and TNF-alpha stimulated by LPS. Overall, MET reduced liver NAFLD but promoted hepatocyte increase in pro-inflammatory cytokines, thus, leading to liver inflammation. (AU)

FAPESP's process: 13/09367-4 - Metabolic effects of doxorubicin on the muscle and adipose tissue: do exercise and metformin reduce the damage?
Grantee:José Cesar Rosa Neto
Support type: Regular Research Grants
FAPESP's process: 15/16777-0 - Physical exercise as a synchronizer of circadian rhythms in cancer
Grantee:Alexandre Abilio de Souza Teixeira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/01409-8 - The caracterization of antiinflammatory effect of palmitoleic acid suplemmentation in hepatic inflammation; The role of PPARs.
Grantee:José Cesar Rosa Neto
Support type: Regular Research Grants