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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Independent regulation of Plasmodium falciparum rif gene promoters

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Author(s):
Duque Araujo, Rosana Beatriz [1] ; Silva, Tatiane Macedo [1] ; Kaiser, Charlotte Sophie [2] ; Leite, Gabriela Fernandes [1] ; Alonso, Diego [3] ; Martins Ribolla, Paulo Eduardo [3] ; Wunderlich, Gerhard [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Av Prof Lineu Prestes 1374, BR-05508000 Sao Paulo, SP - Brazil
[2] Westfalische Wilhelms Univ, Inst Anim Physiol, Schlosspl 8, Munster - Germany
[3] State Univ Sao Paulo, IBB IBTEC, Dept Parasitol, Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 8, JUN 19 2018.
Web of Science Citations: 0
Abstract

All Plasmodium species express variant antigens which may mediate immune escape in the vertebrate host. In Plasmodium falciparum, the rif gene family encodes variant antigens which are partly exposed on the infected red blood cell surface and may function as virulence factors. Not all rif genes are expressed at the same time and it is unclear what controls rif gene expression. In this work, we addressed global rif transcription using plasmid vectors with two drug resistance markers, one controlled by a rif 5' upstream region and the second by a constitutively active promoter. After spontaneous integration into the genome of one construct, we observed that the resistance marker controlled by the rif 5' upstream region was expressed dependent on the applied drug pressure. Then, the global transcription of rif genes in these transfectants was compared in the presence or absence of drugs. The relative transcript quantities of all rif loci did not change profoundly between strains grown with or without drug. We conclude that either there is no crosstalk between rif loci or that the elusive system of allelic exclusion of rif gene transcription is not controlled by their 5' upstream region alone. (AU)

FAPESP's process: 15/17174-7 - Factors influencing expression of variant antigen families var, rif and surf of Plasmodium falciparum, the human malaria parasite
Grantee:Gerhard Wunderlich
Support type: Regular Research Grants
FAPESP's process: 12/23306-5 - Studies on the mechanism of transcriptional control and switching of virulence-associated genes in Plasmodium falciparum
Grantee:Gerhard Wunderlich
Support type: Regular Research Grants