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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

First Chromosomal Analysis in Hepsetidae (Actinopterygii, Characiformes): Insights into Relationship between African and Neotropical Fish Groups

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Author(s):
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Carvalho, Pedro C. [1] ; de Oliveira, Ezequiel A. [1, 2] ; Bertollo, Luiz A. C. [1] ; Yano, Cassia F. [1] ; Oliveira, Claudio [3] ; Decru, Eva [4] ; Jegede, Oladele I. [5] ; Hatanaka, Terumi [1] ; Liehr, Thomas [6] ; Al-Rikabi, Ahmed B. H. [6] ; Cioffi, Marcelo de B. [1]
Total Authors: 11
Affiliation:
[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP - Brazil
[2] Estado Educ Mato Grosso Seduc MT, Cuiaba - Brazil
[3] Univ Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP - Brazil
[4] Royal Museum Cent Africa, Sect Vertebrates Ichthyol, Tervuren - Belgium
[5] Adamawa State Univ, Dept Fisheries & Aquaculture, Mubi - Nigeria
[6] Univ Hosp Jena, Inst Human Genet, Jena - Germany
Total Affiliations: 6
Document type: Journal article
Source: FRONTIERS IN GENETICS; v. 8, DEC 12 2017.
Web of Science Citations: 8
Abstract

Hepsetidae is a small fish family with only the genus Hepsetus, with six described species distributed throughout the South, Central and Western regions of Africa, showing a close relationship with the Alestidae and some Neotropical fish families. However, no cytogenetic information is available for both Hepsetidae and Alestidae species, thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing new cytogenetic data for Hepsetus odoe, including the standard karyotype, C-banding, repetitive DNAs mapping, comparative genomic hybridization (CGH) and whole chromosome painting (WCP), providing chromosomal patterns and subsidies for comparative cytogenetics with other characiform families. Both males and females H. odoe have 2n = 58 chromosomes (10m + 28sm + 20st/a), with most of the C-band positive heterochromatin localized in the centromeric and subtelomeric regions. Only one pair of chromosomes bears proximal 5S rDNA sites in the short arms, contrasting with the 18S rDNA sequences which are located in the terminal regions of four chromosome pairs. Clear interstitial hybridization signals are evidenced for the U1 and U2 snDNA probes, but in only one and two chromosome pairs, respectively. Microsatellite motifs are widely distributed in the karyotype, with exception for the (CGG)(10), (GAA)(10) and (GAG)(10) probes, which highlight conspicuous interstitial signals on an unique pair of chromosomes. Comparative data from conventional and molecular cytogenetics, including CGH and WCP experiments, indicate that H. odoe and some Erythrinidae species, particularly Erythrinus erythrinus, share similar chromosomal sequences suggesting some relatedness among them, although bearing genomic specificities in view of their divergent evolutionary histories. (AU)

FAPESP's process: 16/17556-0 - Evolutionary relationships of the Erythrinidae family (Teleostei, Characiformes). IV: comparative cytogenetics in Hepsetidae
Grantee:Pedro Cristovão Carvalho
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/21411-7 - Karyotype and Sex Chromosomes Evolution in Neotropical Fishes. Part II.
Grantee:Luiz Antonio Carlos Bertollo
Support type: Regular Research Grants
FAPESP's process: 17/08471-3 - Comparative genomic hybridization, microdissection and chromosome painting in Hepsetus akawo (Characiformes, Hepsetidae)
Grantee:Pedro Cristovão Carvalho
Support type: Scholarships abroad - Research Internship - Scientific Initiation
FAPESP's process: 14/26508-3 - Phylogeny of the hyperdiverse order Characiformes (Teleostei: Ostariophysi) using ultraconserved elements
Grantee:Claudio de Oliveira
Support type: Research Projects - Thematic Grants