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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Virulence genes, capsular and plasmid types of multidrug-resistant CTXM(-2,-8,-15) and KPC-2-producing Klebsiella pneumoniae isolates from four major hospitals in Brazil

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Author(s):
Andrade, Leonardo Neves [1] ; Novais, Angela [2] ; Marcato Stegani, Lenita Maria [1] ; Ferreira, Joseane Cristina [1] ; Rodrigues, Carla [2] ; Costa Darini, Ana Lucia [1] ; Peixe, Luisa [2]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, FCFRP, Ribeirao Preto, SP - Brazil
[2] Univ Porto, Fac Farm, Lab Microbiol, UCIBIO REQUIMTE, Porto - Portugal
Total Affiliations: 2
Document type: Journal article
Source: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 91, n. 2, p. 164-168, JUN 2018.
Web of Science Citations: 2
Abstract

We performed a single-month snapshot study of the population diversity of multidrug resistant (MDR) Klebsiella pneumoniae isolates producing carbapenemases and/or extended-spectrum beta-lactamases from four major hospitals in Brazil. Isolates produced diverse ESBL (CTX-M-2, -8, -15, SHV-2), KPC-2 or both (CTX-M-2 and KPC-2), linked to specific genetic backgrounds and plasmids from a few families (IncR, IncFlIk, IncL/M) that were shared among clonal lineages within and between hospitals. A high clonal diversity was identified, among isolates from the same ST (ST11, ST15, ST101 or ST340). Diverse capsular types (n=13 K-types) were identified, most of which linked to specific ST (ST11 and K27 or K64, ST101 and K17, ST340 and KL151, ST15 and K24 or ST17 and KL112). Isolates shared a common set of virulence genes (ureA, fimH, uge, wabG, mrkD, entB) and occasionally ybtS (42%) and kfuBC (18%). Our data suggest intra- and inter-hospital spread of common genetic structures and international MDR K. pneumoniae clones. (C) 2018 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 12/14740-3 - Molecular investigation of Klebsiella pneumoniae isolates and plasmids carrying beta-lactamases encoding genes
Grantee:Ana Lúcia da Costa Darini
Support type: Regular Research Grants
FAPESP's process: 11/08892-2 - Molecular investigation of Klebsiella pneumoniae strains and plasmids carrying beta-lactamases encoding genes
Grantee:Leonardo Neves de Andrade
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/26188-6 - Virulence factors and intraclonal diversity of ESBL and carbapenemase-producing Klebsiella pneumoniae and Escherichia coli clinical isolates
Grantee:Leonardo Neves de Andrade
Support type: Scholarships abroad - Research Internship - Post-doctor