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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae: Perciformes)

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Author(s):
de Campos, Mariana Frias [1, 2] ; Lo Nostro, Fabiana L. [3, 4] ; Da Cuna, Rodrigo H. [3, 4] ; Moreira, Renata Guimardes [1, 2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Ctr Biol Marinha CEBIMar, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Lab Metab & Reprod Organismos Aquat, Sao Paulo - Brazil
[3] Univ Buenos Aires, FCEN, Dept Biodiversidad & Biol Expt, Lab Ecotoxicol Acuat, Buenos Aires, DF - Argentina
[4] UBA, CONICET, IBBEA, Buenos Aires, DF - Argentina
Total Affiliations: 4
Document type: Journal article
Source: General and Comparative Endocrinology; v. 257, n. SI, p. 255-263, FEB 1 2018.
Web of Science Citations: 2
Abstract

The dusky grouper Epinephelus marginatus is a protogynous hermaphrodite fish, that maintains high levels of plasma steroids as juveniles, as substrates for sex inversion. These fish are exposed to marine pollution from oil spills during cargo handling. Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene (Phe), are the main crude oil components and are toxic to fish, acting as endocrine disruptors (ED). This is the first study that investigated impacts of Phe as an ED in E. marginatus juveniles. An in vivo sublethal exposure (96 h) to Phe was carried out at two concentrations (0.1 mg/L and 1 mg/L); exposure to the vehicle (ethanol; ETOH) was also performed. Plasma levels of 17 beta-estradiol (E-2), testosterone (T) and 11-ketotestosterone (11-KT) were measured by ELISA. Gonads, liver and spleen were processed for histological analysis. In an in vitro bioassay, gonad fragments were incubated with Phe (8.91 mg/L) or ETOH. Steroid levels in the culture media were measured by ELISA. The in vivo exposure to Phe triggered an increase of the area of the hepatocytes, increased number of melanomacrophagic centers and hemosiderosis in the spleen; ETOH induced similar effects on spleen. E-2 and T levels did not change in plasma or in vitro media. In plasma, ETOH decreased 11-KT levels. Phenanthrene sharply reduced 11-KT levels in vitro. Although in vivo bioassay results were not unequivocal owing to ethanol effects, Phe might disrupt steroidogenesis in juvenile grouper, possibly causing dysfunctions during sex change and gonadal maturity, considering the importance of 11-KT in developing ovaries. (C) 2017 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 13/50480-9 - Neotropical fish as indicators of aquatic contamination
Grantee:Renata Guimarães Moreira Whitton
Support type: Regular Research Grants