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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

HPV-transformed cells exhibit altered HMGB1-TLR4/MyD88-SARM1 signaling axis

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Morale, Mirian Galliote [1, 2] ; Abjaude, Walason da Silva [3] ; Silva, Aline Montenegro [3] ; Villa, Luisa Lina [4, 2] ; Boccardo, Enrique [3]
Total Authors: 5
[1] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil
[2] ICESP, Ctr Translat Oncol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Med, Dept Radiol & Oncol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 8, FEB 22 2018.
Web of Science Citations: 4

Cervical cancer is one of the leading causes of cancer death in women worldwide. Persistent infection with high-risk human papillomavirus (HPV) types is the main risk factor for the development of cervical cancer precursor lesions. HPV persistence and tumor development is usually characterized by innate immune system evasion. Alterations in Toll-like receptors (TLR) expression and activation may be important for the control of HPV infections and could play a role in the progression of lesions and tumors. In the present study, we analyzed the mRNA expression of 84 genes involved in TLR signaling pathways. We observed that 80% of the differentially expressed genes were downregulated in cervical cancer cell lines relative to normal keratinocytes. Major alterations were detected in genes coding for several proteins of the TLR signaling axis, including TLR adaptor molecules and genes associated with MAPK pathway, NF kappa B activation and antiviral immune response. In particular, we observed major alterations in the HMGB1-TLR4 signaling axis. Functional analysis also showed that HMGB1 expression is important for the proliferative and tumorigenic potential of cervical cancer cell lines. Taken together, these data indicate that alterations in TLR signaling pathways may play a role in the oncogenic potential of cells expressing HPV oncogenes. (AU)

FAPESP's process: 14/21361-4 - Effect of HPV16 oncoproteins on DNA damage repair pathways in human keratinocytes organotypic cultures
Grantee:Aline Montenegro Silva
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/20002-0 - Study of Synthetic Lethality in cells infected with Human Papillomaviruses (HPV)
Grantee:Enrique Mario Boccardo Pierulivo
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 11/14416-9 - Study of E6 and E7 proteins from HPV16 and HPV6 and its effects on Toll like receptors signaling pathways
Grantee:Mirian Galliote Morale
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 08/57889-1 - Institute of Science and Technology to study Diseases Associated with Papillomavirus
Grantee:Luisa Lina Villa
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 08/03232-1 - HPV and tumor microenvironment
Grantee:Luisa Lina Villa
Support Opportunities: Research Projects - Thematic Grants