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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Xenotopic expression of alternative electron transport enzymes in animal mitochondria and their impact in health and disease

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Camargo, Andre F. [1] ; Chioda, Marina M. [1] ; Rodrigues, Ana P. C. [1] ; Garcia, Geovana S. [1] ; McKinney, Emily A. [1] ; Jacobs, Howard T. [2, 3, 4] ; Oliveira, Marcos T. [1]
Total Authors: 7
[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil
[2] Univ Tampere, Inst Biosci & Med Technol, FI-33014 Tampere - Finland
[3] Univ Tampere, Tampere Univ Hosp, FI-33014 Tampere - Finland
[4] Univ Helsinki, Inst Biotechnol, FI-00014 Helsinki - Finland
Total Affiliations: 4
Document type: Review article
Source: Cell Biology International; v. 42, n. 6, SI, p. 664-669, JUN 2018.
Web of Science Citations: 1

The mitochondrial respiratory chain in vertebrates and arthropods is different from that of most other eukaryotes because they lack alternative enzymes that provide electron transfer pathways additional to the oxidative phosphorylation (OXPHOS) system. However, the use of diverse experimental models, such as human cells in culture, Drosophila melanogaster and the mouse, has demonstrated that the transgenic expression of these alternative enzymes can impact positively many phenotypes associated with human mitochondrial and other cellular dysfunction, including those typically presented in complex IV deficiencies, Parkinson's, and Alzheimer's. In addition, these enzymes have recently provided extremely valuable data on how, when, and where reactive oxygen species, considered by many as by-products of OXPHOS, can contribute to animal longevity. It has also been shown that the expression of the alternative enzymes is thermogenic in cultured cells, causes reproductive defects in flies, and enhances the deleterious phenotype of some mitochondrial disease models. Therefore, all the reported beneficial effects must be considered with caution, as these enzymes have been proposed to be deployed in putative gene therapies to treat human diseases. Here, we present a brief review of the scientific data accumulated over the past decade that show the benefits and the risks of introducing alternative branches of the electron transport into mammalian and insect mitochondria, and we provide a perspective on the future of this research field. (AU)

FAPESP's process: 14/02253-6 - Investigating the metabolic alterations caused by the transgenic expression of the mitochondrial alternative oxidase of Ciona intestinalis in Drosophila melanogaster
Grantee:Marcos Túlio de Oliveira
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 17/03806-7 - Evaluation of the thermogenic properties of the mitochondrial alternative oxidase expressed in Drosophila melanogaster
Grantee:Geovana Siqueira Garcia
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 17/02813-0 - Mitochondrial proteomics of Drosophila melanogaster expressing the alternative oxidase under different dietary conditions
Grantee:Marina Minari Chioda
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 15/14547-7 - Effects of AOX expression in mitochondrial DNA helicase mutants of Drosophila melanogaster
Grantee:Ana Paula Campos Rodrigues
Support Opportunities: Scholarships in Brazil - Master