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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Arginine and Polyamines Fate in Leishmania Infection

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Muxel, Sandra M. [1] ; Aoki, Juliana I. [1] ; Fernandes, Juliane C. R. [1] ; Laranjeira-Silva, Maria F. [1] ; Zampieri, Ricardo A. [1] ; Acuna, Stephanie M. [1] ; Muller, Karl E. [2] ; Vanderlinde, Rubia H. [1] ; Floeter-Winter, Lucile M. [1]
Total Authors: 9
[1] Univ Sao Paulo, Inst Biosci, Dept Physiol, Sao Paulo - Brazil
[2] Univ Bergen, Dept Clin Sci, Bergen - Norway
Total Affiliations: 2
Document type: Review article
Web of Science Citations: 12

Leishmania is a protozoan parasite that alternates its life cycle between the sand fly and the mammalian host macrophages, involving several environmental changes. The parasite responds to these changes by promoting a rapid metabolic adaptation through cellular signaling modifications that lead to transcriptional and post-transcriptional gene expression regulation and morphological modifications. Molecular approaches such as gene expression regulation, next-generation sequencing (NGS), microRNA (miRNA) expression profiling, in cell Western blot analyses and enzymatic activity profiling, have been used to characterize the infection of murine BALB/c and C57BL/6 macrophages, as well as the human monocytic cell-lineage THP-1, with Leishmania amazonensis wild type (La-WT) or arginase knockout (La-arg(-)). These models are being used to elucidate physiological roles of arginine and polyamines pathways and the importance of arginase for the establishment of the infection. In this review, we will describe the main aspects of Leishmania-host interaction, focusing on the arginine and polyamines pathways and pointing to possible targets to be used for prognosis and/or in the control of the infection. The parasite enzymes, arginase and nitric oxide synthase-like, have essential roles in the parasite survival and in the maintenance of infection. On the other hand, in mammalian macrophages, defense mechanisms are activated inducing alterations in the mRNA, miRNA and enzymatic profiles that lead to the control of infection. Furthermore, the genetic background of both parasite and host are also important to define the fate of infection. (AU)

FAPESP's process: 16/22896-4 - MicroRNA profile of human macrophages derived from the THP-1 cell line infected with Leishmania (L.) amazonensis
Grantee:Juliane Cristina Ribeiro Fernandes
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/50717-1 - Biochemical, physiological and functional genomic studies of Leishmania-macrophage interaction
Grantee:Lucile Maria Floeter-Winter
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/25942-4 - Establishment profiles expression of miRNA of infected murine macrophages by Leishmania (L.) amazonensis
Grantee:Stephanie Maia Acuna
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/19815-2 - Role of miRNAs in the TLRs-mediated immune response to Leishmania amazonensis infection
Grantee:Sandra Marcia Muxel
Support Opportunities: Regular Research Grants