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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of Curcumin-Nanoemulsion Associated with Photodynamic Therapy in Cervical Carcinoma Cell Lines

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Adum de Matos, Renata Prandini [1] ; Calmon, Marilia Freitas [1] ; Amantino, Camila Fernanda [2, 3] ; Villa, Luisa Lina [4] ; Primo, Fernando Lucas [5, 6] ; Tedesco, Antonio Claudio [2, 3] ; Rahal, Paula [1]
Total Authors: 7
[1] Sao Paulo State Univ UNESP, Inst Biosci Humanities & Exact Sci IBILCE, Dept Biol, Rua Cristovao Colombo, 2265 Bairro Jardim Nazareth, BR-15054010 Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Chem, Ctr Nanotechnol & Tissue Engn Photobiol, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Photomed Res Grp, Fac Philosophy Sci & Letters Ribeirao Preto, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[4] ICESP, Ctr Translat Res Oncol, Av Dr Arnaldo 251, 8 Andar, BR-01246000 Sao Paulo, SP - Brazil
[5] Sao Paulo State Univ UNESP, Fac Pharmaceut Sci Araraquara, Dept Bioproc & Biotechnol, Rodovia Araraquara Jau, Km 01 S-N, BR-14800903 Araraquara, SP - Brazil
[6] Univ Sao Paulo, Dept Radiol & Oncol, Fac Med, Av Dr Arnaldo 251, 8 Andar, BR-01246000 Sao Paulo, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Web of Science Citations: 4

Cervical cancer is the fourth cause of cancer death in women. Curcumin has antineoplastic properties. Furthermore, curcumin may be used as a photosensitizing agent in Photodynamic Therapy. This study aimed to investigate the effects of Photodynamic Therapy in cellular viability using curcumin-nanoemulsion as a photosensitizing drug in cervical carcinoma cell lines. Theempty nanoemulsion presented very lowcytotoxicity in all cell lines analyzed. Additionally, the incubation with curcumin-nanoemulsion at 20.. Mof curcumin showed more than 80% of cell viability for cell lines. Nanoemulsions were shown to be internalized inside cells by fluorescence microscopy and were observed in the intracellular environment for up to 36 hours after incubation with cell lines. In addition, after the Photodynamic Therapy we observed a high phototoxic effect of the curcumin-nanoemulsion with less than 5% of viable cells after irradiation. This was accompanied by an increase in caspase-3/caspase-7 activities after cell treatment with curcumin-nanoemulsion and Photodynamic Therapy, suggesting cell death by apoptosis. We conclude that the curcumin-nanoemulsion formulation behaves as a photosensitizing drug in Photodynamic Therapy and shows potential as an alternative treatment to cervical lesions using an endoscopic diode fiber laser setup for in situ activation or cavity activation using a diffuse fiber delivery system. (AU)

FAPESP's process: 13/04286-6 - Study of the action of curcumin-nanoparticles in HPV-16 positive cervical carcinoma cells
Grantee:Renata Prandini Adum de Matos
Support Opportunities: Scholarships in Brazil - Doctorate