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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis

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Rossoni, Rodnei Dennis [1] ; de Barros, Patricia Pimentel [1] ; de Alvarenga, Janaina Araujo [1] ; Ribeiro, Felipe de Camargo [1] ; Velloso, Marisol dos Santos [1] ; Fuchs, Beth Burgwyn [2] ; Mylonakis, Eleftherios [2] ; Cardoso Jorge, Antonio Olavo [1] ; Junqueira, Juliana Campos [1]
Total Authors: 9
[1] UNESP Univ Estadual Paulista, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos - Brazil
[2] Brown Univ, Rhode Isl Hosp, Alpert Med Sch, Div Infect Dis, Providence, RI 02903 - USA
Total Affiliations: 2
Document type: Journal article
Source: BIOFOULING; v. 34, n. 2, p. 212-225, 2018.
Web of Science Citations: 17

This study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation. The hindrance of biofilm development was characterized by the downregulated expression of C. albicans biofilm-specific genes (ALS3, HWP1, EFG1 and CPH1). L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 demonstrated the ability to exert antifungal activity through the inhibition of C. albicans biofilms. (AU)

FAPESP's process: 13/26820-4 - Galleria mellonella as non-vertebrate host infection model for the study of Porphyromonas gingivalis: analysis of virulence and sensitivity to photodynamic therapy
Grantee:Janaina Araújo de Alvarenga
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/09770-9 - Isolation and characterization of Lactobacillus spp. from oral cavity and its probiotic action in Candida albicans and Streptococcus mutans
Grantee:Juliana Campos Junqueira
Support Opportunities: Regular Research Grants
FAPESP's process: 13/25181-8 - Isolation and characterization of Lactobacillus spp. from oral cavity and their probiotic action in Candida albicans: biofilm formation, infection in invertebrate models and EFG1, HWP1 and ALS1 gene expression
Grantee:Rodnei Dennis Rossoni
Support Opportunities: Scholarships in Brazil - Doctorate