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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Exercise Training Restores the Cardiac Microrna-16 Levels Preventing Microvascular Rarefaction in Obese Zucker Rats

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Fernandes, Tiago [1] ; Casaes, Lilian [1] ; Soci, Ursula [1] ; Silveira, Andre [1] ; Gomes, Joao [1] ; Barretti, Diego [1] ; Roque, Fernanda [1] ; Oliveira, Edilamar [1]
Total Authors: 8
[1] Univ Sao Paulo, Lab Biochem & Mol Biol Exercise, Sch Phys Educ & Sport, Av Prof Mello Moraes 65, BR-05508900 Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Obesity Facts; v. 11, n. 1, p. 15-24, 2018.
Web of Science Citations: 4

Objective: To evaluate the effects of aerobic exercise training (AET) on cardiac miRNA-16 levels and its target gene VEGF related to microvascular rarefaction in obese Zucker rats (OZR). Methods: OZR (n = 11) and lean (L; n = 10) male rats were assigned into 4 groups: OZR, trained OZR (OZRT), L and trained L (LT). Swimming exercise training lasted 60 min, 1x/day/10 weeks, with 4% body weight workload. Cardiac angiogenesis was assessed by histological analysis (periodic acid-Schiff) by calculating the capillary/fiber ratio. The protein expressions of VEGF, VEGFR2, and CD31 were evaluated by western blot. The expression of miRNA-16 was evaluated by real-time PCR. Results: Heart rate decreased in the trained groups compared to sedentary groups. The cardiac capillary/fiber ratio was reduced in OZR compared to L, LT and OZRT groups, indicating that aerobic exercise training (AET) was capable of reversing the microvascular rarefaction in the obese animals. miRNA-16 expression was increased in OZR compared to L, LT and OZRT. In contrast, its target, VEGF protein expression was 24% lower in OZR compared to L group, which has been normalized in OZRT group. VEGFR2 protein expression was increased in trained groups compared to their controls. CD31, a endothelial cells marker, showed increased expression in OZRT compared to OZR, indicating greater vascularization in OZRT group. Conclusion: AET induced cardiac angiogenesis in obese animals. This revascularization is associated with a decrease in miRNA-16 expression permissive for increased VEGF protein expression, suggesting a mechanism for potential therapeutic application in vascular diseases. (C) 2018 The Author(s) Published by S. Karger GmbH, Freiburg (AU)

FAPESP's process: 09/18370-3 - microRNA expression on normotensive rats heart submitted to different aerobic training and the potential to hypertension therapy.
Grantee:Edilamar Menezes de Oliveira
Support type: Regular Research Grants
FAPESP's process: 10/50048-1 - Cellular and functional bases of exercise in cardiovascular diseases
Grantee:Carlos Eduardo Negrão
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/17275-8 - Role of microRNA-205 in the angiogenic deficit mediated by FAK signaling in hypertension: effect of exercise training and perspective of the therapeutic potential
Grantee:Tiago Fernandes
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/50673-4 - A new therapeutic approach to arterial repair and regeneration by exercise training and microRNAs targeted to vasorin
Grantee:Edilamar Menezes de Oliveira
Support type: Regular Research Grants