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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Acute lung injury induced by intestinal ischemia and reperfusion is altered in obese female mice

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Author(s):
Fantozzi, Evelyn Thais [1] ; Rodrigues-Garbin, Sara [1] ; Ricardo-da-Silva, Fernanda Yamamoto [2] ; Oliveira-Filho, Ricardo Martins [1] ; Spina, Domenico [3] ; Tavares-de-Lima, Wothan [1] ; Riffo-Vasquez, Yanira [3]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Med Sch, Heart Inst InCor, Lab Cardiovasc Surg & Physiopathol Circulat LIM 1, Sao Paulo - Brazil
[3] Kings Coll London, Inst Pharmaceut Sci, Sackler Inst Pulm Pharmacol, 150 Stamford St, London SE1 9NH - England
Total Affiliations: 3
Document type: Journal article
Source: Pulmonary Pharmacology & Therapeutics; v. 49, p. 54-59, APR 2018.
Web of Science Citations: 1
Abstract

Rational: Acute lung injury (ALI) is a common complication after intestinal ischemia and reperfusion (I/R) injury that can lead to acute respiratory distress syndrome (ARDS). We have previously demonstrated that females are protected against lung damage induced by intestinal I/R through an estrogen mediated mechanism. Objectives: To investigate the effect of obesity on ALI induced by intestinal I/R in female mice. Methods: C57B1/6 female mice were fed with a standard low-fat diet (SD) or a high-fat diet (HFD) for 9 weeks. Intestinal I/R injury was induced by a 45 min occlusion of the mesenteric artery followed by 2 and 24 h of reperfusion. Results: Significant increase in lung myeloperoxidase expression (MPO) and neutrophil numbers of SD and HFD mice occurred at 2 h and 24 h of reperfusion. Furthermore, HFD mice presented a significant increase in lung eosinophil peroxidase (EPO) expression and eosinophil numbers compared to SD mice. Lung wet/dry weight ratio was significantly greater in HFD mice at 2 and 24 h of reperfusion, accompanied by a significant increase in the expression of inducible NO in the lung tissue and a significant decrease in arterial oxygen saturation at 24 h of reperfusion relative to SD mice. Conclusion: Obesity predisposes female mice to increased pulmonary oedema and deterioration in gas exchange, which is accompanied by an increase in iNOS expression in the lung. (AU)

FAPESP's process: 10/51330-2 - Obesity and sex hormones in lung injury
Grantee:Wothan Tavares de Lima
Support type: Regular Research Grants
FAPESP's process: 12/50550-4 - Obesity and sex hormones in lung injury
Grantee:Wothan Tavares de Lima
Support type: Regular Research Grants