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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lithobates catesbeianus (American Bullfrog) oocytes: a novel heterologous expression system for aquaporins

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Author(s):
Kabutomori, Jessica [1] ; Beloto-Silva, Olivia [1, 2] ; Geyer, R. Ryan [3] ; Musa-Aziz, Raif [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo - Brazil
[2] Paulista Univ UNIP, Dept Hlth Sci, BR-06542001 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05513970 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOLOGY OPEN; v. 7, n. 4 APR 2018.
Web of Science Citations: 0
Abstract

Xenopus laevis oocytes are a valuable tool for investigating the function of membrane proteins. However, regulations around the world, specifically in Brazil, render the import of Xenopus laevis frogs impractical, and, in some cases, impossible. Here, as an alternative, we evaluate the usefulness of the North American aquatic bullfrog Lithobates catesebeianus, which is commercially available in Brazil, for the heterologous expression of aquaporin (AQP) proteins. We have developed a method that combines a brief collagenase treatment and mechanical defolliculation for isolating individual oocytes from Lithobates ovaries. We find that they have a similar size, shape, and appearance to Xenopus oocytes and can tolerate and survive following injections with cRNA or water. Furthermore, surface biotinylation, western blot analysis, and measurements of osmotic water permeability (P-f) show that Lithobates oocytes can express AQPs to the plasma membrane and significantly increase the P-f of the oocytes. In fact, the P-f values are similar to historical values gathered from Xenopus oocytes. Due to the presence of a mercury sensitive cysteine (Cys or C) in the throat of the water channel, the Pf of oocytes expressing human (h) AQP1, hAQP1(FLAG) {[} FLAG, short protein tag (DYKDDDDK) added to the N-terminus of AQP1], hAQP8, and rat (r)AQP9 was inhibited with the mercurial compound p-chloromercuribenzene sulfonate (pCMBS), whereas AQPs lacking this Cys-hAQP1(C189S) mutant {[}residueCys 189 was replaced by a serine (Ser or S)] and hAQP7 -were mercury insensitive. Contrary to previous studies with Xenopus oocytes, rAQP3 was also found to be insensitive tomercury, which is consistent with the mercury-sensitive Cys (Cys 11) being located intracellularly. Thus, we consider Lithobates oocytes to be a readily accessible system for the functional expression and study of membrane proteins for international researchers who do not currently have access to Xenopus oocytes. (AU)

FAPESP's process: 13/11364-3 - New model system for the heterologous expression and functional evaluation of ammonia (NH3/NH4 +) transporters using Rana catesbeiana oocytes: an alternative to the Xenopus laevis system
Grantee:Raif Musa Aziz
Support type: Regular Research Grants
FAPESP's process: 13/23087-4 - Molecular and functional study of membrane transporters
Grantee:Gerhard Malnic
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/10780-3 - Oxidation and aggregation of human superoxide dismutase 1 resulting from its bicarbonate-dependent peroxidase activity
Grantee:Robert Ryan Geyer
Support type: Scholarships in Brazil - Post-Doctorate