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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Induction of systemic inflammation by hyaluronan and hsp70 in women with pre-eclampsia

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Author(s):
Romao-Veiga, Mariana [1] ; Matias, Mariana Leticia [2] ; Ribeiro, Vanessa Rocha [2] ; Nunes, Priscila Rezeck [2] ; Borges, Vera Therezinha M. [2] ; Peracoli, Jose Carlos [2] ; Peracoli, Maria Terezinha S. [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ, UNESP, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Gynaecol & Obstet, Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CYTOKINE; v. 105, p. 23-31, MAY 2018.
Web of Science Citations: 6
Abstract

Pre-eclampsia (PE) is a human pregnancy syndrome with abnormal activation of the innate immune response. The study evaluated the involvement of molecular structures called damage-associated molecular patterns (DAMPs), such as hyaluronan (HA) and heat shock proteins (Hsp) on NLRP1 and NLRP3 inflammasomes activation in peripheral blood monocytes. Twenty pre-eclamptic women, 20 normotensive pregnant women (NT) and 20 non-pregnant women (NP) were studied. Enzyme immunoassay was employed for the determination of HA, Hsp70 and High mobility group Box 1 (HMGB1) in plasma, as well as for the detection of Interleukin-1 beta (IL-1 beta), IL-18 and tumor necrosis factor alpha (TNF-alpha) in the supernatant of monocytes cultured with or without HA and Hsp70. The inflammasomes induction was evaluated by the quantification of mRNA for NLRP1, NLRP3, caspase-1, IL-1 beta, IL-18, HMGB1 and TNF-alpha by qPCR in monocyte culture. The results showed significantly higher plasma levels of HA, Hsp70 and HMGB1 in pre-eclamptic women than in NT and NP women. Monocytes from women with PE showed endogenous activation of NLRP1 and NLRP3 inflammasomes, and expressed high amounts of IL-1 beta, IL-18, HMGB1 and TNF-alpha. The stimulation of monocytes with HA increased the gene expression of NLRP1, NLRP3, caspase-1, TNF-alpha, HMGB1 and IL-18 and the production of IL-1 beta in preeclamptic women. Monocytes cultured with Hsp70 produced elevated levels of IL-1 beta and TNF-alpha through a mechanism independent of inflammasomes activation. These results suggest the participation of these DAMPs in the systemic inflammatory response that is characteristic of PE. (AU)

FAPESP's process: 12/24697-8 - Inflammasome and autophagy involvement in the pathophysiology of preeclampsia
Grantee:Maria Terezinha Serrão Peraçoli
Support type: Regular Research Grants
FAPESP's process: 13/00534-5 - The role of inflammasome in the innate and adaptive immunity in pregnant women with preeclampsia
Grantee:Mariana Romão Veiga
Support type: Scholarships in Brazil - Doctorate