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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

SPM-1-producing Pseudomonas aeruginosa ST277 clone recovered from microbiota of migratory birds

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Martins, Willames M. B. S. [1] ; Narciso, Ana Clara [1] ; Cayo, Rodrigo [1] ; Santos, Stefanie Vanessa [1] ; Fehlberg, Lorena C. C. [1] ; Ramos, Patricia Locosque [2] ; da Cruz, Joao Batista [3] ; Gales, Ana Cristina [1]
Total Authors: 8
[1] Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Internal Med, Div Infect Dis, Lab Alerta, Sao Paulo - Brazil
[2] Fundacao Parque Zool Sao Paulo, Dept Pesquisas Aplicadas, Sao Paulo, SP - Brazil
[3] FPZSP, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 1

The production of Sao Paulo metallo-O-lactamase (SPM-1) is the most common carbapenem resistance mechanism detected among multidrug-resistant Pseudomonas aeruginosa clinical isolates in Brazil. Dissemination of SPM-1-producing P. aeruginosa has been restricted to the nosocomial settings, with sporadic reports of environmental isolates due to contamination by hospital sewage. Herein, we described the detection and molecular characterization of SPM-1-producing P. aeruginosa recovered from the microbiota of migratory birds in Brazil. Three hundred gram-negative bacilli were recovered from cloacal and choanal swabs of Dendrocygna viduata during a surveillance study for detection of carbapenem-resistant isolates. All isolates were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. MICs were determined by agar dilution, except for polymyxin B. Antibiotic resistance genes were detected by polymerase chain reaction (PCR) followed by DNA sequencing. Transcriptional levels of oprD and efflux system encoding genes were also carried out by quantitative real-time PCR. Nine imipenem-resistant P. aeruginosa isolates were recovered with 7 of them carrying bla(SPM-1). Additional resistance genes (rmtD-1, bla(OXA-56), aacA4, and aac(6')-Ib-cr) were also detected in all 9 isolates. The SPM-1-producing isolates showed high MICs for all beta-lactams, fluoroquinolones, and aminoglycosides, being susceptible only to polymyxin B. Interestingly, all isolates showed the same PFGE pattern and belonged to ST277. Overexpression of MexXY-OprM and MexAB-OprM was observed in those isolates that did not harbor bla(SPM-1). Our results suggest that migratory birds might have played a role in the dissemination of SPM-1-producing P. aeruginosa within the Brazilian territory. (C) 2017 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 14/12224-3 - Evaluation of the mechanisms of resistance to beta-lactams among Burkholderia cenocepacia clinical isolates recovered from bloodstream infections
Grantee:Lorena Cristina Corrêa Fehlberg
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/02258-6 - Role of the Migratory Bird Dendrocygna viduata on the Dissemination of Antimicrobial-Resistant Gram-negative Rods
Grantee:Ana Cristina Gales
Support Opportunities: Regular Research Grants