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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of intracameral ascorbic acid on the corneal endothelium of dogs undergoing phacoemulsification

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Padua, Ivan R. M. [1] ; Valdetaro, Gisele P. [1] ; Lima, Tiago B. [1] ; Kobashigawa, Karina K. [1] ; Silva, Paloma E. S. [2] ; Aldrovani, Marcela [1] ; Padua, Pedro P. M. [3] ; Laus, Jose L. [1]
Total Authors: 8
[1] UNESP, Dept Small Anim Med & Surg, Fac Agr & Vet Sci, Ophthalmol Unit, FCAV, Jaboticabal, SP - Brazil
[2] UNESP, Anesthesiol Unit, Dept Small Anim Med & Anim Reprod, Fac Agr & Vet Sci, FCAV, Jaboticabal, SP - Brazil
[3] Clin Protectora Anim, Dept Small Anim Med, Bogota - Colombia
Total Affiliations: 3
Document type: Journal article
Source: VETERINARY OPHTHALMOLOGY; v. 21, n. 2, p. 151-159, MAR 2018.
Web of Science Citations: 1

ObjectiveCataracts are the most common ocular disorder in dogs. Phacoemulsification is the preferred treatment method among ophthalmologists, but the cellularity of the endothelium must be considered for its success, as endothelial lesions may produce permanent corneal decompensation. The objective of this study was to evaluate the effects of intracameral ascorbic acid, a known antioxidant, on the corneal endothelium of dogs undergoing phacoemulsification. Animal studiedIn all, 40 eyes from 20 dogs, males and females from 7 to 12 years of age, were assessed for mature cataracts. ProceduresTwo groups were formed (n = 20): Group 1 (G1) received a balanced salt solution (BSS), whereas Group 2 (G2) received sterile ascorbic acid diluted in a BSS, at a final concentration of 0.001 m ascorbic acid. The corneal endothelium was assessed via non-contact specular microscopy at multiple time points before and after phacoemulsification. Cell density (cells/mm(2)) and area (mm(2)), corneal thickness (mm), hexagonality, and the coefficient of variation of cell size were all assessed. P values equal to or less than 0.05 were considered significant. ResultsWith respect to the density of endothelial cells, both groups showed losses, but they were less severe in G2. There were no differences in corneal thickness. Hexagonality decreased significantly in the postoperative period in G1. Also in G1, the coefficient of variation of cell size increased significantly. ConclusionAccording to the results obtained, ascorbic acid minimizes cellular losses in the corneal endothelium. (AU)

FAPESP's process: 11/20041-8 - Effects of ascorbic acid on the corneal endothelium and on tumor necrosis factor-alpha (tnf-±) in dogs undergoing phacoemulcification
Grantee:Ivan Ricardo Martinez Padua
Support Opportunities: Scholarships in Brazil - Doctorate