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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development and Validation of a Rapid and Reliable RP-HPLC-PDA Method for the Quantification of Six Diterpenes in Copaifera duckei, Copaifera reticulata and Copaifera multijuga Oleoresins

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Carneiro, Luiza J. [1] ; Bianchi, Thamires C. [1] ; da Silva, Jonas J. M. [2] ; Oliveira, Larissa C. [1] ; Borges, Carly H. G. [1] ; Lemes, Danieli C. [1] ; Bastos, Jairo K. [2] ; Veneziani, Rodrigo C. S. [1] ; Ambrosio, Sergio R. [1]
Total Authors: 9
[1] Univ Franca, Nucleo Pesquisa Ciencias Exatas & Tecnol, Av Dr Armando de Salles Oliveira 201, BR-14404600 Franca, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 29, n. 4, p. 729-737, APR 2018.
Web of Science Citations: 2

Oleoresins from Copaifera species are extensively used in folk medicine in Brazil, which are employed mainly in the production of cosmetic formulations in Brazil, North America and Europe. Considering the lack of validated analytical methods for the analyses of diterpenes in Copaifera oleoresins, it was developed a validated and reliable reversed-phase high-performance liquid chromatography with photodiode array detection (RP-HPLC-PDA) method for the analysis of six diterpenes, including: (-)-polyalthic acid; (13E)-ent-labda-7,13-dien-15-oic acid; ent-8(17) -labden-15,18-dioic acid; (-)-copalic acid; (-)-3 beta-acetoxycopalic acid and (-)-3 beta-hydroxycopalic acid. These compounds were isolated from C. duckei, C. reticulata and C. multijuga oleoresins by chromatographic means. The analytical curves were linear with regression coefficients (r(2)) between 0.9903 and 0.9999. The limits of detection (LOD) and quantification (LOQ) values were 0.35 to 3.09 mu g mL(-1) and 1.05 to 9.36 mu g mL(-1), respectively. The method also displayed good precision and accuracy. The developed analytical method is reliable and a useful tool for the analysis of Copaifera oleoresin and its products. (AU)

FAPESP's process: 16/01232-0 - Copaifera oleoresins: development and validation of a HPLC-DAD analytical method and phytochemical study and in vitro antitumor evaluation of the main secondary metabolites obtained
Grantee:Luiza Junqueira Carneiro
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 14/03092-6 - Chemical validation and in vitro antitumor activity of secondary metabolites obtained from the oleoresin of Copaifera multijuga
Grantee:Luiza Junqueira Carneiro
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
Grantee:Jairo Kenupp Bastos
Support Opportunities: Research Projects - Thematic Grants