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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum

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Arya, Sunil K. [1] ; Zhurauski, Pavel [1] ; Jolly, Pawan [1] ; Batistuti, Marina R. [2] ; Mulato, Marcelo [2] ; Estrela, Pedro [1]
Total Authors: 6
[1] Univ Bath, Dept Elect & Elect Engn, Bath BA2 7AY, Avon - England
[2] Univ Sao Paulo, Dept Phys, BR-14040901 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BIOSENSORS & BIOELECTRONICS; v. 102, p. 106-112, APR 15 2018.
Web of Science Citations: 26

We report the development of a simple and powerful capacitive aptasensor for the detection and estimation of human epidermal growth factor receptor 2 (HER2), a biomarker for breast cancer, in undiluted serum. The study involves the incorporation of interdigitated gold electrodes, which were used to prepare the electrochemical platform. A thiol terminated DNA aptamer with affinity for HER2 was used to prepare the bio-recognition layer via self-assembly on interdigitated gold surfaces. Non-specific binding was prevented by blocking free spaces on surface via starting block phosphate buffer saline-tween20 blocker. The sensor was characterized using cyclic voltammetry, electrochemical impedance spectroscopy (EIS), atomic force microscopy and contact angle studies. Non-Faradic EIS measurements were utilized to investigate the sensor performance via monitoring of the changes in capacitance. The aptasensor exhibited logarithmically detection of HER2 from 1 pM to 100 nM in both buffer and undiluted serum with limits of detection lower than 1 pM. The results pave the way to develop other aptamer-based biosensors for protein biomarkers detection in undiluted serum. (AU)

FAPESP's process: 15/14403-5 - Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers
Grantee:Marina Ribeiro Batistuti
Support type: Scholarships abroad - Research Internship - Doctorate