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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Neurogenic mediators contribute to local edema induced by Micrurus lemniscatus venom

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Casais-e-Silva, Luciana Lyra [1] ; Teixeira, Catarina [2]
Total Authors: 2
[1] Univ Fed Bahia, Lab Neuroimmunoendocrinol & Toxinol, Dept Bioregulat, Inst Hlth Sci ICS, Salvador, BA - Brazil
[2] Butantan Inst, Lab Pharmacol, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS Neglected Tropical Diseases; v. 11, n. 11 NOV 2017.
Web of Science Citations: 1

Background/Aims Micrurus is one of the four snake genera of medical importance in Brazil. Coral snakes have a broad geographic distribution from the southern United States to Argentina. Micrurine envenomation is characterized by neurotoxic symptoms leading to dyspnea and death. Moreover, various local manifestations, including edema formation, have been described in patients bitten by different species of Micrurus. Thus, we investigated the ability of Micrurus lemniscatus venom (MLV) to induce local edema. We also explored mechanisms underlying this effect, focussing on participation of neuropeptides and mast cells. Methodology/Principal findings Intraplantar injection of MLV (1-10 mu g/paw) in rats caused dose-and time-dependent edema with a peak between 15 min and 1 h after injection. MLV also induced degranulation of peritoneal mast cells (MCs). MC depletion by compound 48/80 markedly reduced MLV-induced edema. Pre-treatment (30 min) of rats with either prometazine, a histamine H1 receptor antagonist or methysergide, a nonselective 5-HT receptor antagonist, reduced MLV-induced edema. However, neither thioperamide, a histamine H-3/H-4 receptor antagonist, nor co-injection of MLV with HOE-140, a BK2 receptor antagonist, altered the response. Depletion of neuropeptides by capsaicin or treatment of animals with NK1- and NK2-receptor antagonists (SR 140333 and SR 48968, respectively) markedly reduced MLV-induced edema. Conclusions/Significance In conclusion, MLV induces paw edema in rats by mechanisms involving activation of mast cells and substance P-releasing sensory C-fibers. Tachykinins NKA and NKB, histamine, and serotonin are major mediators of the MLV-induced edematogenic response. Targeting mast cell-and sensory C-fiber-derived mediators should be considered as potential therapeutic approaches to interrupt development of local edema induced by Micrurus venoms. (AU)

FAPESP's process: 14/18549-1 - Characterization of the effects of a snake venom Phospholipase A2 (MT-III) on smooth muscle vascular cells in culture: formation of foam cells and mechanisms involved in this effect
Grantee:Catarina de Fatima Pereira Teixeira
Support Opportunities: Regular Research Grants