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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens

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Author(s):
Caiaffa, Karina Sampaio [1] ; Massunari, Loiane [1] ; Danelon, Marcelle [2] ; Abuna, Gabriel Flores [3] ; Lombardo Bedran, Telma Blanca [4, 5] ; Santos-Filho, Norival Alves [6] ; Palomari Spolidorio, Denise Madalena [4] ; Vizoto, Natalia Leal [7] ; Cilli, Eduardo Maffud [6] ; Duque, Cristiane [2]
Total Authors: 10
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Restorat Dent, Aracatuba - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Pediat Dent & Publ Hlth, Aracatuba - Brazil
[3] Univ Campinas UNICAMP, Sch Dent, Dept Restorat Dent, Piracicaba - Brazil
[4] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara - Brazil
[5] Nove Julho Univ, Dept Periodontol, Sao Paulo - Brazil
[6] Sao Paulo State Univ UNESP, Inst Chem, Dept Biochem & Chem Technol, Araraquara - Brazil
[7] Univ Campinas UNICAMP, Sch Dent, Dept Oral Diag, Piracicaba - Brazil
Total Affiliations: 7
Document type: Journal article
Source: BIOFOULING; v. 33, n. 10, p. 807-818, 2017.
Web of Science Citations: 5
Abstract

This study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3-1C(V) and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT. The minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) of the peptides and CHX were determined against oral pathogens associated with endodontic infections. Enterococcus faecalis and Streptococcus mutans biofilms were cultivated in bovine dentin blocks, exposed to different concentrations of the most efficient antimicrobial peptide and analyzed by confocal laser scanning microscopy. CHX and peptides affected the metabolism of L-929 at concentrations > 31.25 and 500 mu g ml(-1), respectively. Among the peptides, KR-12-a5 inhibited growth of both the microorganisms tested with the lowest MIC/ MBC/MFC values. In addition, KR-12-a5 significantly reduced E. faecalis and S. mutans biofilms inside dentin tubules. In conclusion, KR-12-a5 is a non-cytotoxic agent with potent antimicrobial and antibiofilm activity against oral pathogens associated with endodontic infections. (AU)

FAPESP's process: 12/15346-7 - Antimicrobial peptides: dimerization effects and encapsulation in NRLb biomembrane
Grantee:Eduardo Maffud Cilli
Support type: Regular Research Grants
FAPESP's process: 13/24606-5 - Antimicrobial effect of cationic peptides analogs on endodontic microrganisms and its influence on gene expression of dentin mineralization markers
Grantee:Karina Sampaio Caiaffa
Support type: Scholarships in Brazil - Master