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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

DNA polymeric films as a support for cell growth as a new material for regenerative medicine: Compatibility and applicability

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Author(s):
Jayme, Cristiano Ceron [1] ; de Paula, Leonardo Barcelos [1] ; Rezende, Nayara [1] ; Calori, Italo Rodrigo [1] ; Franchi, Leonardo Pereira [1] ; Tedesco, Antonio Claudio [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Photobiol & Photomed Res Grp, Dept Chem, Ctr Nenotechnol & Tissue Engn, Ave Bandeirantes, 3900, BR-14040901 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Experimental Cell Research; v. 360, n. 2, p. 404-412, NOV 15 2017.
Web of Science Citations: 5
Abstract

DNA polymeric films (DNA-PFs) are a promising drug delivery system (DDS) in modem medicine. In this study, we evaluated the growth behavior of oral squamous cell carcinoma (OSCC) cells on DNA-PFs. The morphological, biochemical, and cytometric features of OSCC cell adhesion on DNA-PFs were also assessed. An initial, temporary alteration in cell morphology was observed at early time points owing to the inhibition of cell attachment to the film, which then returned to a normal morphological state at later time points. MIT and resazurin assays showed a moderate reduction in cell viability related to increased DNA concentration in the DNAP-Fs. Flow cytometry studies showed low cytotoxicity of DNA-PFs, with cell viabilities higher than 90% in all the DNA-PFs tested. Flow cytometric cell cycle analysis also showed average cell cycle phase distributions at later time points, indicating that OSCC cell growth is maintained in the presence of DNA-PFs. These results show high biocompatibility of DNA-PFs and suggest their use in designing ``dressing material,{''} where the DNA film acts as a support for cell growth, or with incorporation of active or photoactive compounds, which can induce tissue regeneration and are useful to treat many diseases, especially oral cancer. (AU)

FAPESP's process: 13/50181-1 - Use of drugs containing nanocarriers with photosensitizers and/or other active compounds applied to cell therapy and treatment of central nervous system disorders
Grantee:Antonio Claudio Tedesco
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/11870-9 - Sensitizing cancer cell lines to photodynamic therapy through inhibition of protein APE1
Grantee:Leonardo Pereira Franchi
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/18684-9 - Use of drugs containing nanocarriers with photosensitizers and/or other active compounds applied to cell therapy and treatment of central nervous system disorders
Grantee:Leonardo Barcelos de Paula
Support type: Scholarships in Brazil - Post-Doctorate