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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Baccharis dracunculifolia DC (Asteraceae) selectively modulates the effector functions of human neutrophils

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Author(s):
Figueiredo-Rinhel, Andrea S. G. [1] ; de Melo, Lamartine L. [2, 1] ; Bortot, Leandro O. [1] ; Santos, Everton O. L. [2, 1] ; Andrade, Micassio F. [2, 1] ; Azzolini, Ana Elisa C. S. [1] ; Kabeya, Luciana M. [1] ; Caliri, Antonio [1] ; Bastos, Jairo K. [3] ; Lucisano-Valim, Yara Maria [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Pharmacy and Pharmacology; v. 69, n. 12, p. 1829-1845, DEC 2017.
Web of Science Citations: 2
Abstract

ObjectivesTo examine whether the hydroalcoholic extract from Baccharis dracunculifolia leaves (BdE) modulates the human neutrophil oxidative metabolism, degranulation, phagocytosis and microbial killing capacity. MethodsIn-vitro assays based on chemiluminescence, spectrophotometry, flow cytometry and polarimetry were used, as well as docking calculations. Key findingsAt concentrations that effectively suppressed the neutrophil oxidative metabolism elicited by soluble and particulate stimuli (<10 g/ml), without clear signs of cytotoxicity, BdE (1) inhibited NADPH oxidase and myeloperoxidase activity; (2) scavenged H2O2 and HOCl; (3) weakly inhibited phagocytosis; and (4) did not affect neutrophil degranulation and microbial killing capacity, the expression levels of TLR2, TLR4, FcRIIa, FcRIIIb and CR3 and the activity of elastase and lysozyme. Caffeic acid, one of the major B. dracunculifolia secondary metabolites, did not inhibit phagocytosis but interfered in the myeloperoxidase-H2O2-HOCl system by scavenging H2O2 and HOCl, and interacting with the catalytic residues His-95, Arg-239 and Gln-91. ConclusionsBdE selectively modulates the effector functions of human neutrophils, inhibits the activity of key enzymes and scavenges physiological oxidant species. Caffeic acid contributes to lower the levels of oxidant species. Our findings help to unravel the mechanisms by which these natural products exert immunomodulatory action towards neutrophils. (AU)

FAPESP's process: 12/23541-4 - In vitro evaluation of antioxidant and anti-inflammatory derivatives 3-phenylcoumarins in human neutrophils stimulated and in animal models of arthritis.
Grantee:Micássio Fernandes de Andrade
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/19504-0 - Study of the antioxidant activity mechanism of Baccharis dracunculifolia(Asteraceae) in neutrophils and evaluation of this effect on rheumatoid arthritis model
Grantee:Andréa Silva Garcia de Figueiredo Rinhel
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/20810-7 - Systematic study of the molecular mechanisms involved in the inhibition of the oxidative metabolism of the stimulated neutrophils by the Baccharis dracunculifolia, 3-phenylcoumarin derivatives and 3,5,7-triidroxiflavonaimplications on the RA therapeutics
Grantee:Yara Maria Lucisano Valim
Support Opportunities: Regular Research Grants
FAPESP's process: 13/00927-7 - Molecular dynamics simulations for the identification of bioactive molecules: inhibitors of the interaction between the dengue virus envelope glycoprotein and cellular C-type lectins
Grantee:Leandro Oliveira Bortot
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)