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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Environmental DNA characterization of amphibian communities in the Brazilian Atlantic forest: Potential application for conservation of a rich and threatened fauna

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Sasso, Thais [1] ; Lopes, Carla M. [2, 3] ; Valentini, Alice [4] ; Dejean, Tony [4] ; Zarnudio, Kelly R. [5] ; Haddad, Celio F. B. [2, 3] ; Martins, Marcio [1]
Total Authors: 7
[1] Univ Sao Paulo, Inst Biociencias, Dept Ecol, Rua Matao, Trav 321, BR-05508090 Sao Paulo, SP - Brazil
[2] Univ Estadual Paulista UNESP, Dept Zool, Inst Biociencias, Campus Rio Claro, BR-13506900 Rio Claro, SP - Brazil
[3] Ctr Aquicultura CAUNESP, Campus Rio Claro, BR-13506900 Rio Claro, SP - Brazil
[4] SPYGEN, Savoie Technolac, BP 274, F-73375 Le Bourget Du Lac - France
[5] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 - USA
Total Affiliations: 5
Document type: Journal article
Source: Biological Conservation; v. 215, p. 225-232, NOV 2017.
Web of Science Citations: 2

The Brazilian Atlantic forest is a highly threatened biodiversity hotspot, harboring one of the highest levels of amphibian species richness in the world. Amphibian conservation in Neotropical biomes is critical because freshwater ecosystems typically experience sharp declines in biodiversity before much is known about species that depend on those environments. Environmental DNA (eDNA) analysis is a new approach for monitoring aquatic organisms and provides valuable information on species occurrence in freshwater ecosystems. Here, we assess community diversity in Neotropical streams using eDNA metabarcoding. We compare data from a five-year traditional field survey with results from a short-term eDNA analysis in four streams of the Atlantic forest of southeastern Brazil. We recorded 19 species over 5 years using visual-acoustic methods, of which 10 species are associated with the streams during at least one life stage (egg, tadpole or post-metamorphic). We were able to detect nine of the latter species using eDNA metabarcoding from water samples collected over 4 days. Amphibian community composition as measured by both methods showed high similarity in three streams, but was not perfect, as eDNA failed to detect known species in a fourth stream. Furthermore, in one stream we detected through eDNA metabarcoding a species (Aplastodiscus eugenioi) found only once during the 5-year traditional survey in that stream. Also, three species (Cycloramphus boraceiensis, Hylodes asper, and Hylodes phyllodes) with the highest dependence on aquatic habitat showed the highest number of positive PCR detections on eDNA samples. Our results showed that eDNA metabarcoding can be a useful tool to assess community diversity in tropical streams, and although not perfect in detection, this method can potentially improve our ability to conserve Neotropical amphibians. (AU)

FAPESP's process: 16/14054-3 - Environmental DNA applied to monitoring and conservation studies of anurans in the Brazilian Atlantic Forest
Grantee:Carla Martins Lopes
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/50741-7 - Diversity and conservation of Brazilian amphibians
Grantee:Célio Fernando Baptista Haddad
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 14/06795-8 - Detection of stream amphibians in Brazilian Atlantic Forest using environmental DNA
Grantee:Thais Sasso Lopes
Support Opportunities: Scholarships in Brazil - Master