Lopes, Carla M.
Zarnudio, Kelly R.
Haddad, Celio F. B.
Total Authors: 7
 Univ Sao Paulo, Inst Biociencias, Dept Ecol, Rua Matao, Trav 321, BR-05508090 Sao Paulo, SP - Brazil
 Univ Estadual Paulista UNESP, Dept Zool, Inst Biociencias, Campus Rio Claro, BR-13506900 Rio Claro, SP - Brazil
 Ctr Aquicultura CAUNESP, Campus Rio Claro, BR-13506900 Rio Claro, SP - Brazil
 SPYGEN, Savoie Technolac, BP 274, F-73375 Le Bourget Du Lac - France
 Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 - USA
Total Affiliations: 5
Web of Science Citations:
The Brazilian Atlantic forest is a highly threatened biodiversity hotspot, harboring one of the highest levels of amphibian species richness in the world. Amphibian conservation in Neotropical biomes is critical because freshwater ecosystems typically experience sharp declines in biodiversity before much is known about species that depend on those environments. Environmental DNA (eDNA) analysis is a new approach for monitoring aquatic organisms and provides valuable information on species occurrence in freshwater ecosystems. Here, we assess community diversity in Neotropical streams using eDNA metabarcoding. We compare data from a five-year traditional field survey with results from a short-term eDNA analysis in four streams of the Atlantic forest of southeastern Brazil. We recorded 19 species over 5 years using visual-acoustic methods, of which 10 species are associated with the streams during at least one life stage (egg, tadpole or post-metamorphic). We were able to detect nine of the latter species using eDNA metabarcoding from water samples collected over 4 days. Amphibian community composition as measured by both methods showed high similarity in three streams, but was not perfect, as eDNA failed to detect known species in a fourth stream. Furthermore, in one stream we detected through eDNA metabarcoding a species (Aplastodiscus eugenioi) found only once during the 5-year traditional survey in that stream. Also, three species (Cycloramphus boraceiensis, Hylodes asper, and Hylodes phyllodes) with the highest dependence on aquatic habitat showed the highest number of positive PCR detections on eDNA samples. Our results showed that eDNA metabarcoding can be a useful tool to assess community diversity in tropical streams, and although not perfect in detection, this method can potentially improve our ability to conserve Neotropical amphibians. (AU)