Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Melatonin and IL-25 modulate apoptosis and angiogenesis mediators in metastatic (CF-41) and non-metastatic (CMT-U229) canine mammary tumour cells

Full text
Gelaleti, G. B. [1, 2] ; Borin, T. F. [3] ; Maschio-Signorini, L. B. [2] ; Moschetta, M. G. [2] ; Hellmen, E. [4] ; Viloria-Petit, A. M. [5] ; Zuccari, D. A. P. C. [1, 2]
Total Authors: 7
[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Programa Posgrad Genet, IBILCE, Sao Jose Do Rio Preto - Brazil
[2] Fac Med Sao Jose do Rio Preto FAMERP, LIMC, Sao Jose Do Rio Preto - Brazil
[3] Augusta Univ, Georgia Canc Ctr, Tumor Imaging Angiogenesis Lab, Augusta, GA - USA
[4] Swedish Univ Agr Sci, Fac Vet Med, Dept Anat Physiol & Biochem, Uppsala - Sweden
[5] Univ Guelph, Dept Biomed Sci, Ontario Vet Coll, Room 3647, 50 Stone Rd East, Guelph, ON N1G 2W1 - Canada
Total Affiliations: 5
Document type: Journal article
Source: VETERINARY AND COMPARATIVE ONCOLOGY; v. 15, n. 4, p. 1572-1584, DEC 2017.
Web of Science Citations: 2

Background: Melatonin has oncostatic actions and IL-25 is active in inflammatory processes that induce apoptosis in tumor cells Aim: The aim of this study was to evaluate melatonin and IL-25 in metastatic (CF-41) and non-metastatic (CMT-U229) canine mammary tumor cells cultured as monolayers and tridimensional structures. Materials and Methods: The cells were treated with melatonin, IL-25 and IL-17B silencing gene and performed cell viability, gene and protein expression of caspase-3 and VEGFA (Vascular endothelial growth factor A) and an apoptosis membrane protein array. Results: Treatment with 1 mM of melatonin reduced cell viability of both tumor cell lines, all treatments alone and combined significantly increased caspase-3 cleaved and proteins involved in the apoptotic pathway and reduced pro-angiogenic VEGFA, confirming the effectiveness of these potential promising treatments. Conclusion: This is the first study evaluating the potential use of these strategies in CF-41 and CMT-U229 cell lines and together encourages subsequent in vitro and in vivo studies for further exploration of clinical applications. (AU)

FAPESP's process: 12/02128-1 - Modulation of Interleukin 17B and 25 Activity Associated with Melatonin as Inductor of Apoptosis in Cell Culture of Mammary Neoplasms
Grantee:Gabriela Bottaro Gelaleti
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/06098-0 - Modulation of interleukin 17b and 25 activity associated with melatonin as inductor of apoptosis in cell culture of mammary neoplasms
Grantee:Debora Aparecida Pires de Campos Zuccari
Support type: Regular Research Grants