Tryptophan and kynurenine determination in human h... - BV FAPESP
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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Tryptophan and kynurenine determination in human hair by liquid chromatography

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Author(s):
Dario, Michelli F. [1] ; Freire, Thamires Batello [1] ; Sales de Oliveira Pinto, Claudineia Parecida [1] ; Aurora Prado, Maria Segunda [1] ; Baby, Andre R. [1] ; Velasco, Maria Valeria R. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1065, p. 59-62, OCT 15 2017.
Web of Science Citations: 5
Abstract

Tryptophan, an amino acid found in hair proteinaceous structure is used as a marker of hair photodegradation. Also, protein loss caused by several chemical/physical treatments can be inferred by tryptophan quantification. Kynurenine is a photo-oxidation product of tryptophan, expected to be detected when hair is exposed mainly to UVB (290-320 nm) radiation range. Tryptophan from hair is usually quantified directly as a solid or after alkaline hydrolysis, spectrofluorimetrically. However, these types of measure are not sufficiently specific and present several interfering substances. Thus, this work aimed to propose a quantification method for both tryptophan and kynurenine in hair samples, after alkali hydrolysis process, by using high-performance liquid chromatography (HPLC) with fluorimetric and UV detection. The tryptophan and kynurenine quantification method was developed and validated. Black, white, bleached and dyed (blond and auburn) hair tresses were used in this study. Tryptophan and kynurenine were separated within similar to 9 min by HPLC. Both black and white virgin hair samples presented similar concentrations of tryptophan, while bleaching caused a reduction in the tryptophan content as well as dyeing process. Unexpectedly, UV/vis radiation did not promote significantly the conversion of tryptophan into its photo-oxidation product and consequently, kynurenine was not detected. Thus, this works presented an acceptable method for quantification of tryptophan and its photooxidation metabolite kynurenine in hair samples. Also, the results indicated that bleaching and dyeing processes promoted protein/amino acids loss but tryptophan is not extensively degraded in human hair by solar radiation. (AU)

FAPESP's process: 12/04435-9 - Gelatin particle systems containing flavonoid: development, characterization and application in sunscreen formulations
Grantee:André Rolim Baby
Support Opportunities: Regular Research Grants
FAPESP's process: 13/16070-8 - Nanotechnological bioactive hair conditioner to protect hair from ultraviolet radiation
Grantee:Maria Valeria Robles Velasco
Support Opportunities: Regular Research Grants
FAPESP's process: 13/16265-3 - Development and evaluation of the effectiveness of bioactive cationic nanoemulsion in protecting hair photo-oxidative damage
Grantee:Michelli Ferrera Dario
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)