Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

3-Dimensional X-ray microtomography methodology for characterization of monolithic stationary phases and columns for capillary liquid chromatography - A tutorial

Full text
da Silva, Carla G. A. [1, 2] ; Grespan Bottoli, Carla Beatriz [1] ; Collins, Carol H. [1]
Total Authors: 3
[1] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil
[2] Univ Fed Mato Grosso, Dept Chem, BR-78060900 Cuiaba - Brazil
Total Affiliations: 2
Document type: Review article
Source: Analytica Chimica Acta; v. 991, p. 30-45, OCT 23 2017.
Web of Science Citations: 0

In this tutorial we describe a fast, nondestructive, three-dimensional (3-D) view approach to be used in morphology characterization of capillary monoliths and columns by reconstruction from X-ray microtomography (XMT) obtained by acquiring projection images of the sample from a number of different directions. The method comprises imaging acquisition, imaging reconstruction using specific algorithms and imaging analysis by generation of a 3-D image of the sample from radiographic images. The 3-D images show the morphological data for bulk macropore space and skeleton connectivity of the monoliths and were compared with other images from imaging techniques such as scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and with chromatographic performance. The 3-D XMT methodology is applicable for organic and inorganic capillary chromatographic monolithic materials and it allows the acquisition of many hundreds (in our case 1001 projections) of longitudinal and cross-sectional images in a single session, resolving morphological details with a 3D-view of the monolithic structure, inclusive inside the column in a sectional structure with volume (three dimensions) when compared to the sectional structure area (with only two dimensions) when using SEM and FESEM techniques. (C) 2017 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/23518-2 - Preparation of monolithic capillary columns based on poly(methyloctylsiloxane) thermally immobilized onto silica and mixed oxides for use in capillary liquid chromatography
Grantee:Carla Grazieli Azevedo da Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/25933-0 - Preparation of silica and mixed oxides monoliths for use in capillary liquid chromatography
Grantee:Carla Beatriz Grespan Bottoli
Support Opportunities: Regular Research Grants
FAPESP's process: 08/57805-2 - Institute of Bioanalytics
Grantee:Lauro Tatsuo Kubota
Support Opportunities: Research Projects - Thematic Grants