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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of gene expression levels for cytokines in ocular toxoplasmosis

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Author(s):
Maia, M. M. ; Meira-Strejevitch, C. S. ; Pereira-Chioccola, V. L. ; de Hippolito, D. D. C. ; Silva, V. O. ; Brandao de Mattos, C. C. ; Frederico, F. B. ; Siqueira, R. C. ; de Mattos, L. C. ; Grp, FAMERP IAL Toxoplasma Res
Total Authors: 10
Document type: Journal article
Source: PARASITE IMMUNOLOGY; v. 39, n. 10 OCT 2017.
Web of Science Citations: 5
Abstract

This study evaluated levels for mRNA expression of 7 cytokines in ocular toxoplasmosis. Peripheral blood mononuclear cells (PBMC) of patients with ocular toxoplasmosis (OT Group, n=23) and chronic toxoplasmosis individuals (CHR Group, n=9) were isolated and stimulated in vitro with T. gondii antigen. Negative controls (NC) were constituted of 7 PBMC samples from individuals seronegative for toxoplasmosis. mRNA expression for cytokines was determined by qPCR. Results showed a significant increase in mRNA levels from antigen stimulated PBMCs derived from OT Group for expressing IL-6 (at P<.005 and P<.0005 for CHR and NC groups, respectively), IL-10 (at P<.0005 and P<.005 for CHR and NC groups, respectively) and TGF- (at P<.005) for NC group. mRNA levels for TNF- and IL-12 were also upregulated in patients with OT compared to CHR and NC individuals, although without statistical significance. Additionally, mRNA levels for IL-27 and IFN- in PBMC of patients with OT were upregulated in comparison with NC individuals. Differences between OT and NC groups were statistically significant at P<.05 and P<.0005, respectively. (AU)

FAPESP's process: 13/15879-8 - Toxoplasma gondii infection: serological and molecular characterization in patients attended in public health service (SUS)
Grantee:Fernando Henrique Antunes Murata
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/04803-6 - Cytokine expression in the modulation of cutaneous leishmaniasis and its interaction with clinical, epidemiological and laboratory findings
Grantee:Daise Damaris Carnietto de Hippólito
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/10050-5 - Serological, molecular and images in patients with active lesions or acute relapse of ocular infection by Toxoplasma gondii: a study follow-up
Grantee:Mariana Previato
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/25650-8 - Class II HLA genes (DRB1 and DQB1) as risck factors for ocular toxoplasmosis
Grantee:Luiz Carlos de Mattos
Support type: Regular Research Grants
FAPESP's process: 14/09496-1 - New diagnostic strategies employed in symptomatic toxoplasmosis
Grantee:Vera Lúcia Pereira Chioccola
Support type: Regular Research Grants