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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

microRNA159-targeted SIGAMYB transcription factors are required for fruit set in tomato

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Author(s):
da Silva, Eder Marques ; Ferreira e Silva, Geraldo Felipe ; Bidoia, Debora Brussolo ; Azevedo, Mariana da Silva ; de Jesus, Frederico Almeida ; Pino, Lilian Ellen ; Pereira Peres, Lazaro Eustaquio ; Carrera, Esther ; Lopez-Diaz, Isabel ; Silveira Nogueira, Fabio Tebaldi
Total Authors: 10
Document type: Journal article
Source: Plant Journal; v. 92, n. 1, p. 95-109, OCT 2017.
Web of Science Citations: 8
Abstract

The transition from flowering to fruit production, namely fruit set, is crucial to ensure successful sexual plant reproduction. Although studies have described the importance of hormones (i.e. auxin and gibberellins) in controlling fruit set after pollination and fertilization, the role of microRNA-based regulation during ovary development and fruit set is still poorly understood. Here we show that the microRNA159/GAMYB1 and -2 pathway (the miR159/GAMYB1/2 module) is crucial for tomato ovule development and fruit set. MiR159 and SlGAMYBs were expressed in preanthesis ovaries, mainly in meristematic tissues, including developing ovules. SlMIR159-overexpressing tomato cv. Micro-Tom plants exhibited precocious fruit initiation and obligatory parthenocarpy, without modifying fruit shape. Histological analysis showed abnormal ovule development in such plants, which led to the formation of seedless fruits. SlGAMYB1/2 silencing in SlMIR159-overexpressing plants resulted in misregulation of pathways associated with ovule and female gametophyte development and auxin signalling, including AINTEGUMENTA-like genes and the miR167/SlARF8a module. Similarly to SlMIR159-overexpressing plants, SlGAMYB1 was downregulated in ovaries of parthenocarpic mutants with altered responses to gibberellins and auxin. SlGAMYBs likely contribute to fruit initiation by modulating auxin and gibberellin responses, rather than their levels, during ovule and ovary development. Altogether, our results unveil a novel function for the miR159-targeted SlGAMYBs in regulating an agronomically important trait, namely fruit set. (AU)

FAPESP's process: 15/17892-7 - Functional study of the miR159- and miR319-regulated pathways during tomato flower and fruit development
Grantee:Fabio Tebaldi Silveira Nogueira
Support type: Regular Research Grants
FAPESP's process: 15/14286-9 - Over-expression of SlyMIR159 alters flower development and promoters parthenocarpic fruit formation in tomato
Grantee:Eder Marques da Silva
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 13/02469-6 - Contribution of the LeGAMYB1/microRNA 159 pathway in the regulation of development of lateral organs and fruit formation in tomato (Solanum Lycopersicum) cv micro
Grantee:Eder Marques da Silva
Support type: Scholarships in Brazil - Doctorate