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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cytotoxicity and genotoxicity of stilbene derivatives in CHO-K1 and HepG2 cell lines

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Author(s):
Mizuno, Cassia Suemi ; Ampomaah, Winnifred ; Mendonca, Fernanda Ribeiro ; Andrade, Gabriela Carvalho ; Nazare da Silva, Ariel Maria ; Goulart, Mirian Oliveira ; dos Santos, Raquel Alves
Total Authors: 7
Document type: Journal article
Source: GENETICS AND MOLECULAR BIOLOGY; v. 40, n. 3, p. 656-664, JUL-SEP 2017.
Web of Science Citations: 3
Abstract

The cytotoxicity and genotoxicity of the stilbenes (E)-methyl-4-(3-5-dimethoxystyryl) benzoate (ester), (E)-4-(3-5-dimethoxystyryl) aniline (amino), (Z)-1,3-dimethoxy-5-(4-methoxystyryl) benzene (cis-TMS) and (E)-1,3-dimethoxy5-(4-methoxystyryl) benzene (trans-TMS) were investigated in this work. Structural modifications of resveratrol, a naturally occurring stilbene, have been previously performed, including the replacement of hydroxyl by different functional groups. Such modifications resulted in significant improvement of target-specific effects on cell death and antiproliferative responses. The parameters were evaluated using XTT assay, clonogenic survival assay and the cytokinesis-block micronucleus assay in CHO-K1 and HepG2 cell lines. The results showed that cis-TMS is approximately 250-fold more cytotoxic than the amino and ester, and 128-fold more cytotoxic than trans-TMS. When genotoxicity was evaluated, only the trans-TMS did not significantly increase the frequency of micronucleus (MN). While the cis-TMS induced a mean of 5.2 and 5.9 MN/100 cells at 0.5 mu M in CHO-K1 and HepG2, respectively, the amino and ester induced 3.1 and 3.6 MN/100 cells at 10 mu M in CHO-K1, respectively, and 3.5 and 3.8 in HepG2. Trans-TMS is genotoxic only in HepG2 cells. Based on these results, the cis-TMS was the most cytotoxic and genotoxic compound in both cell lines. (AU)

FAPESP's process: 14/12465-0 - Assessment of DNA damage response pathways modulated by structuraly-modified stilbenes
Grantee:Raquel Alves dos Santos
Support type: Regular Research Grants