P2X7 receptor drives Th1 cell differentiation and ... - BV FAPESP
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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

P2X7 receptor drives Th1 cell differentiation and controls the follicular helper T cell population to protect against Plasmodium chabaudi malaria

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de Salles, Erika Machado ; de Menezes, Maria Nogueira ; Siqueira, Renan ; da Silva, Henrique Borges ; Amaral, Eduardo Pinheiro ; Castillo-Mendez, Sheyla Ines ; Cunha, Isabela ; Cassado, Alexandra dos Anjos ; Vieira, Flavia Sarmento ; Nicholas Olivieri, David ; Tadokoro, Carlos Eduardo ; Alvarez, Jose Maria ; Coutinho-Silva, Robson ; D'Imperio-Lima, Maria Regina
Total Authors: 14
Document type: Journal article
Source: PLOS PATHOGENS; v. 13, n. 8 AUG 2017.
Web of Science Citations: 15
Abstract

A complete understanding of the mechanisms underlying the acquisition of protective immunity is crucial to improve vaccine strategies to eradicate malaria. However, it is still unclear whether recognition of damage signals influences the immune response to Plasmodium infection. Adenosine triphosphate (ATP) accumulates in infected erythrocytes and is released into the extracellular milieu through ion channels in the erythrocyte membrane or upon erythrocyte rupture. The P2X7 receptor senses extracellular ATP and induces CD4 T cell activation and death. Here we show that P2X7 receptor promotes T helper 1 (Th1) cell differentiation to the detriment of follicular T helper (Tfh) cells during blood-stage Plasmodium chabaudi malaria. The P2X7 receptor was activated in CD4 T cells following the rupture of infected erythrocytes and these cells became highly responsive to ATP during acute infection. Moreover, mice lacking the P2X7 receptor had increased susceptibility to infection, which correlated with impaired Th1 cell differentiation. Accordingly, IL-2 and IFN gamma secretion, as well as T-bet expression, critically depended on P2X7 signaling in CD4 T cells. Additionally, P2X7 receptor controlled the splenic Tfh cell population in infected mice by promoting apoptotic-like cell death. Finally, the P2X7 receptor was required to generate a balanced Th1/Tfh cell population with an improved ability to transfer parasite protection to CD4-deficient mice. This study provides a new insight into malaria immunology by showing the importance of P2X7 receptor in controlling the fine-tuning between Th1 and Tfh cell differentiation during P. chabaudi infection and thus in disease outcome. (AU)

FAPESP's process: 10/51150-4 - Animal model for analysis of the pathogenicity of Mycobacterium bovis strains and evaluation of cellular and humoral immune responses against pathogenic isolates
Grantee:Maria Regina D'Império Lima
Support Opportunities: Regular Research Grants
FAPESP's process: 15/20432-8 - Intervention in signaling pathways associated with the recognition of cellular damage to reduce the pathology of severe forms of malaria and tuberculosis
Grantee:Maria Regina D'Império Lima
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 11/11053-2 - Effects of purinergic signaling during acute and chronic infection by Plasmodium chabaudi AS
Grantee:Érika Machado de Salles
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/07140-2 - Role of inflammasomas in the pathogenesis of tuberculosis caused by hypervirulent clinical isolates of mycobacteria
Grantee:Maria Regina D'Império Lima
Support Opportunities: Regular Research Grants