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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microbial diversity of a full-scale UASB reactor applied to poultry slaughterhouse wastewater treatment: integration of 16S rRNA gene amplicon and shotgun metagenomic sequencing

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Delforno, Tiago Palladino ; Lacerda Junior, Gileno Vieira ; Noronha, Melline F. ; Sakamoto, Isabel K. ; Varesche, Maria Bernadete A. ; Oliveira, Valeria M.
Total Authors: 6
Document type: Journal article
Source: MICROBIOLOGYOPEN; v. 6, n. 3 JUN 2017.
Web of Science Citations: 9

The 16S rRNA gene amplicon and whole-genome shotgun metagenomic (WGSM) sequencing approaches were used to investigate wide-spectrum profiles of microbial composition and metabolic diversity from a full-scale UASB reactor applied to poultry slaughterhouse wastewater treatment. The data were generated by using MiSeq 2 x 250 bp and HiSeq 2 x 150 bp Illumina sequencing platforms for 16S amplicon and WGSM sequencing, respectively. Each approach revealed a distinct microbial community profile, with Pseudomonas and Psychrobacter as predominant genus for the WGSM dataset and Clostridium and Methanosaeta for the 16S rRNA gene amplicon dataset. The virome characterization revealed the presence of two viral families with Bacteria and Archaea as host, Myoviridae, and Siphoviridae. A wide functional diversity was found with predominance of genes involved in the metabolism of acetone, butanol, and ethanol synthesis; and one-carbon metabolism (e.g., methanogenesis). Genes related to the acetotrophic methanogenesis pathways were more abundant than methylotrophic and hydrogenotrophic, corroborating the taxonomic results that showed the prevalence of the acetotrophic genus Methanosaeta. Moreover, the dataset indicated a variety of metabolic genes involved in sulfur, nitrogen, iron, and phosphorus cycles, with many genera able to act in all cycles. BLAST analysis against Antibiotic Resistance Genes Database (ARDB) revealed that microbial community contained 43 different types of antibiotic resistance genes, some of them were associated with growth chicken promotion (e.g., bacitracin, tetracycline, and polymyxin). (AU)

FAPESP's process: 13/09386-9 - Integrated metatranscriptomic and metagenomic analyses of Caatinga soil and genes prospection involved on the liginin degradation.
Grantee:Gileno Vieira Lacerda Júnior
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/16426-0 - Metagenomics of microbiota of anaerobic reactors applied in anionic surfactant degradation in commercial laundry wastewater
Grantee:Tiago Palladino Delforno
Support Opportunities: Scholarships in Brazil - Post-Doctorate