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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

15d-PGJ(2) as an endoplasmic reticulum stress manipulator in multiple myeloma in vitro and in vivo

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Sperandio, Marcelo ; Demasi, Ana Paula D. ; Martinez, Elizabeth F. ; Saad, Sara O. ; Pericole, Fernando V. ; Vieira, Karla P. ; Freitas, Nadir S. ; Araujo, Vera C. ; Brown, Amy Louise ; Clemente-Napimoga, Juliana Trindade ; Napimoga, Marcelo Henrique
Total Authors: 11
Document type: Journal article
Source: Experimental and Molecular Pathology; v. 102, n. 3, p. 434-445, JUN 2017.
Web of Science Citations: 4

Multiple myeloma (MM) is characterised by intense protein folding and, consequently endoplasmic reticulum (ER) stress. The prostaglandin 15d-PGJ(2) is able to raise oxidative stress levels within the cell and potentially trigger cell death. The aim of this study was to evaluate the antineoplastic effect of 15d-PGJ(2) on MM in vitro and in vivo via ER and oxidative stress pathways. MM.1R and MM.1S cell lines were treated with 15d-PGJ(2) at 1-10 mu M and evaluated with regard to proliferation, mRNA expression of PRDX1, PRDX4, GRP78, GRP94, CHOP, BCL-2 and BAX. Stress data was validated via oxidized glutathione assays. MM. 1R cells were inoculated into NOD/SCID mice, which were subsequently treated daily with 15d-PGJ(2) at 4 mg/kg or vehicle (control), with tumour volume being monitored for 14 days. 15d-PGJ(2) reduced cell proliferation, induced cell death and apoptosis at 5 mu M and 10 mu M and Stress-related genes were upregulated at the same doses. Oxidized glutathione levels were also increased. 15d-PGJ(2) at 4 mg/kg in vivo halted tumour growth. In conclusion, 15d-PGJ(2) induced myeloma cell death via ER stress in vitro. 15d-PGJ(2) in vivo also inhibited tumour growth. (AU)

FAPESP's process: 13/02347-8 - The effect of 15d-PGJ2 on multiple myeloma cell line development in vitro and in vivo
Grantee:Marcelo Sperandio
Support Opportunities: Scholarships in Brazil - Post-Doctoral