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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bromelain Loading and Release from a Hydrogel Formulated Using Alginate and Arabic Gum

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Ataide, Janaina Artem ; Cefali, Leticia Caramori ; Rebelo, Marcia de Araujo ; Spir, Livia Genovez ; Tambourgi, Elias Basile ; Jozala, Angela Faustino ; Chaud, Marco Vinicius ; Silveira, Edgar ; Gu, Xiaochen ; Mazzola, Priscila Gava
Total Authors: 10
Document type: Journal article
Source: Planta Medica; v. 83, n. 10, p. 870-876, JUL 2017.
Web of Science Citations: 6

An ideal wound dressing ensures a moist environment around the wound area and absorbs exudates from the wound surface. Topical application of bromelain to incised wounds has been shown to reprogram the wound microenvironment to promote effective tissue repair. Combining the characteristics of hydrogels and bromelain is therefore of great interest. Herein, we describe the development of a hydrogel, formulated using alginate and Arabic gum, for bromelain loading and release. The hydrogel formulation was evaluated using response surface methodology, considering the pH value and the concentration of alginate and Arabic gum. Bromelain loading and release were evaluated based on passive diffusion. Differential scanning calorimetry and Fourier transform infrared spectroscopy were performed to confirm bromelain immobilization in the hydrogel. The final hydrogel formulation had a swelling ratio of 227% and incorporated 19% of bromelain from a bromelain solution. Bromelain immobilization in the hydrogel was the result of hydrogen bond formation and was optimal at 4 degrees C after 4 h of contact. This evidence suggests that bromelain entrapment into a hydrogel is a promising strategy for the development of wound dressings that support the debridement of burns and wounds. (AU)

FAPESP's process: 14/26304-9 - Evaluation of skin permeation of bromelain using in vitro diffusion experiment
Grantee:Janaína Artem Ataide
Support type: Scholarships abroad - Research Internship - Scientific Initiation
FAPESP's process: 11/23076-7 - Bromelain extraction from pineapple residues using ethanol precipitation and its application in polymeric hydrogel
Grantee:Janaína Artem Ataide
Support type: Scholarships in Brazil - Scientific Initiation