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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sulfate-Binding Protein (Sbp) from Xanthomonas citri: Structure and Functional Insights

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Pereira, Cristiane Tambascia ; Roesler, Cassia ; Faria, Jessica Nascimento ; Fessel, Melissa Regina ; Balan, Andrea
Total Authors: 5
Document type: Journal article
Source: MOLECULAR PLANT-MICROBE INTERACTIONS; v. 30, n. 7, p. 578-588, JUL 2017.
Web of Science Citations: 3

The uptake and transport of sulfate in bacteria is mediated by an ATP-binding cassette transporter (ABC transporter) encoded by sbpcysUWA genes, whose importance has been widely demonstrated due to their relevance in cysteine synthesis and bacterial growth. In Xanthomonas citri, the causative agent of canker disease, the expression of components from this ABC transporter and others related to uptake of organic sulfur sources has been shown during in vitro growth cultures. In this work, based on gene reporter and proteomics analyses, we showed the activation of the promoter that controls the sbpcysUWA operon in vitro and in vivo and the expression of sulfate-binding protein (Sbp), a periplasmic-binding protein, indicating that this protein plays an important function during growth and that the transport system is active during Citrus sinensis infection. To characterize Sbp, we solved its three-dimensional structure bound to sulfate at 1.14 angstrom resolution and performed biochemical and functional characterization. The results revealed that Sbp interacts with sulfate without structural changes, but the interaction induces a significant increasing of protein thermal stability. Altogether, the results presented in this study show the evidence of the functionality of the ABC transporter for sulfate in X. citri and its relevance during infection. (AU)

FAPESP's process: 13/09172-9 - Structural and funcional characterization of the sulfate ABC transporter from Xanthomonas axonopodis pv. citri and related enzymes
Grantee:Andrea Balan Fernandes
Support Opportunities: Regular Research Grants
FAPESP's process: 13/16094-4 - Functional and structural studies about the sulfate ABC transporter in Xanthomonas citri
Grantee:Cristiane Tambascia Pereira
Support Opportunities: Scholarships in Brazil - Doctorate