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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The effects of calorie-matched high-fat diet consumption on spontaneous physical activity and development of obesity

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Moretto, Thais Ludmilla ; Benfato, Izabelle Dias ; de Carvalho, Francine Pereira ; Barthichoto, Marcela ; Le Sueur-Maluf, Luciana ; Machado de Oliveira, Camila Aparecida
Total Authors: 6
Document type: Journal article
Source: Life Sciences; v. 179, p. 30-36, JUN 15 2017.
Web of Science Citations: 7

Aims: To characterize the effects of a calorie matched high-fat diet (HFD) on spontaneous physical activity (SPA), body weight, inflammatory status and expression of genes related to energy homeostasis in hypothalamus of mice. Main methods: C57BI/6 mice (n = 5 per group) were fed a control diet (16.5% calories from fat) - control group (C), or a calorie matched HFD (60% calories from fat). We evaluated, periodically, body weight and SPA by infrared beam sensors and, at the end of the 12th week, we verified blood glucose levels, fat pads weight, plasma insulin, TNF-alpha and 1L-6 by ELISA and the hypothalamic expression of 84 genes related to energy homeostasis, by quantitative real-time PCR array. Key findings: Isocaloric HFD reduced SPA already in the first 48 h and SPA was kept lower in the HFD compared to C throughout. These changes resulted in an increase in body weight, adiposity, TNF-alpha and IL-6, blood glucose and hyperinsulinemia in the HFD group when compared to the C group. Expression of the Agrp, Bdnf, Adra2b and Pyy genes were altered in the hypothalamus of HFD-fed mice, highlighting the downregulation of Bdnf, key regulator of energy homeostasis. Significance: Dietary macronutrient distribution plays an important part in energy homeostasis that goes beyond its energy content. Despite calorie-matched, the HFD led to increased body weight and adiposity due to decreased SPA, highlighting the key role of SPA on energy balance. The changes in hypothalamic gene expression seem to underlie the reduction in SPA caused by HFD. (C) 2017 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 11/05932-3 - Molecular mechanisms involved in the modulation of spontaneous physical activity and development of obesity
Grantee:Camila Aparecida Machado de Oliveira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/01624-8 - Effects of different diets (low protein and high fat diet) on spontaneous physical activity and hypothalamic expression of genes related to energy homeostasis in mice
Grantee:Thais Ludmilla Moretto
Support type: Scholarships in Brazil - Master