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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Calcium and magnesium ions modulate the oligomeric state and function of mitochondrial 2-Cys peroxiredoxins in Leishmania parasites

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Author(s):
Morais, Mariana A. B. ; Giuseppe, Priscila O. ; Souza, Tatiana A. C. B. ; Castro, Helena ; Honorato, Rodrigo V. ; Oliveira, Paulo S. L. ; Netto, Luis E. S. ; Tomas, Ana M. ; Murakami, Mario T.
Total Authors: 9
Document type: Journal article
Source: Journal of Biological Chemistry; v. 292, n. 17, p. 7023-7039, APR 28 2017.
Web of Science Citations: 1
Abstract

Leishmania parasites have evolved a number of strategies to cope with the harsh environmental changes during mammalian infection. One of these mechanisms involves the functional gain that allows mitochondrial 2-Cys peroxiredoxins to act as molecular chaperones when forming decamers. This function is critical for parasite infectivity in mammals, and its activation has been considered to be controlled exclusively by the enzyme redox state under physiological conditions. Herein, we have revealed that magnesium and calcium ions play a major role in modulating the ability of these enzymes to act as molecular chaperones, surpassing the redox effect. These ions are directly involved in mitochondrial metabolism and participate in a novel mechanism to stabilize the decameric form of 2-Cys peroxiredoxins in Leishmania mitochondria. Moreover, we have demonstrated that a constitutively dimeric Prx1m mutant impairs the survival of Leishmania under heat stress, supporting the central role of the chaperone function of Prx1m for Leishmania parasites during the transition from insect to mammalian hosts. (AU)

FAPESP's process: 11/10248-4 - Cloning, expression, purification and structural characterization of the protein tryparedoxin peroxidase from Leishmania braziliensis
Grantee:Mariana Abrahão Bueno de Morais
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/24134-3 - Functional and structural studies of peroxiredoxin I from Leishmania braziliensis and Homo sapiens aiming to understand the oligomerization and catalysis mechanisms
Grantee:Mariana Abrahão Bueno de Morais
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 15/05851-4 - Functional characterization of the mitochondrial peroxiredoxin I from Leishmania braziliensis evaluating the peroxidase and chaperone activity
Grantee:Mariana Abrahão Bueno de Morais
Support type: Scholarships abroad - Research Internship - Doctorate (Direct)
FAPESP's process: 10/51730-0 - Functional and structural studies of protein kinases involved in cancer and neglected diseases: towards the development of new inhibitors
Grantee:Jörg Kobarg
Support type: Regular Research Grants