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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of bisphosphonates on osteogenesis and osteoclastogenesis signaling during the endochondral ossification of growing rats

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Author(s):
Rezende, Eloiza ; Bradaschia-Correa, Vivian ; Siviero, Fabio ; Ambrosio, Lucas M. B. ; Arana-Chavez, Victor E.
Total Authors: 5
Document type: Journal article
Source: Cell and Tissue Research; v. 368, n. 2, p. 287-300, MAY 2017.
Web of Science Citations: 1
Abstract

Osteoclasts and chondroclasts are necessary, during endochondral ossification, for the resorption of primary bone and calcified cartilage septa, respectively. The bisphosphonates inhibit mineralized tissue resorption by various mechanisms according to the different types of this drug, which can affect bone remodeling during skeletal growth. The objective of the present study is to analyze the way that alendronate (ALN) and etidronate (ETN) can affect osteoclastogenesis and bone formation during endochondral ossification of the long bones of growing rats. Newborn Wistar rats were treated daily with ETN, ALN, or sterile saline solution (control) for 21 days. Their femur and tibiae epiphyses were radiographed and analyzed by light, scanning and transmission electron microscopy. The expression of genes related to osteogenesis and to osteoclast differentiation and activity were analyzed by real-time quantitative polymerase chain reaction. The ETN group presented reduced body weight, disorganized growth plate and an extended area of cartilage in the ossification zone with little bone matrix; in the ALN group, this area was not altered. The ALN presented latent TRAP-positive cells, whereas in the ETN group, they were activated. The expression of NF kappa B1 and 2, OPG, Spp1 and Runx2 in the ossification zone was reduced by both bisphosphonates. RANKL expression was reduced by ETN, whereas ALN decreased the expression of RANK. The results also indicated that, in addition to the anti-resorptive effect of the drugs, disturbances in bone deposition occurred concomitantly with the reduced expression of osteogenesis-related genes. (AU)

FAPESP's process: 10/07509-8 - "IN VIVO" AND IN PRIMARY CULTURE STUDY OF THE EFFECT ON BISPHOSPHONATES ON CLASTIC CELLS DURING THE ENDOCHONDRAL OSSIFICATION OF RAT KNEES
Grantee:Eloiza de Rezende
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/02240-9 - Analysis of the differentiation and activity of clastic cells cultured over bone, dentin and enamel and treated with bisphosphonates
Grantee:Victor Elias Arana-Chavez
Support Opportunities: Regular Research Grants