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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Efficient and new method for Tectona grandis in vitro regeneration

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Tambarussi, Evandro V. ; Rogalski, Marcelo ; Galeano, Esteban ; Brondani, Gilvano Ebling ; de Martin, Valentina de Fatima ; da Silva, Lucas Americo ; Carrer, Helaine
Total Authors: 7
Document type: Journal article
Source: Crop Breeding and Applied Biotechnology; v. 17, n. 2, p. 124-132, APR 2017.
Web of Science Citations: 1

The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA(3). With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA(3) during regeneration, mg L-1) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA(3) during regeneration, mg L-1) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species. (AU)

FAPESP's process: 08/00046-2 - Engineering plant fatty acid biosynthesis by chloroplast transformation
Grantee:Marcelo Rogalski
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/06299-8 - Transcriptional profile analysis of lignified tissues in Tectona grandis L. f
Grantee:Esteban Galeano Gómez
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 07/05795-0 - Development of biotechnological methods for micropropagation, regeneration and genetic transformation of teak (Tectona grandis L. f.) to resistance for Hyblaea puera
Grantee:Evandro Vagner Tambarussi
Support Opportunities: Scholarships in Brazil - Master