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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)


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Rizek, Camila F. ; Luiz, Andre Machado ; de Assis, Gracilene Ramos ; Costa, Silvia Figueiredo ; Levin, Anna Sara ; Lopes, Marta Heloisa
Total Authors: 6
Document type: Journal article
Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 58, 2016.
Web of Science Citations: 1

SUMMARY Neisseria meningitidis is a cause of several life-threatening diseases and can be a normal commensal in the upper respiratory tract of healthy carriers. The carrier rate is not well established especially because there is no standard method for the isolation of N. meningitidis. Therefore, the aim of this study was to compare identification methods for the carrier state. Two swabs were collected from 190 volunteers: one was cultured and the other had DNA extracted directly from the sample. The Polymerase Chain Reaction (PCR) was performed to determine species and serogroups and compared the results between the methods. PCR for species determination used two pairs of primers and when there was only one amplicon, it was sequenced. The culture technique was positive in 23 (12.1%) subjects while the direct extraction method was positive in 132 (69.5%), p < 0.001. Among the 135 subjects with positive N. meningitides tests, 88 (65.2%) were serogroup C; 3 (2.2%) serogroup B; 5 (3.7%) were positive for both serogroup B and C, and 39 (28.9%) did not belong to any of the tested serogroups. In this study, PCR from DNA extracted directly from swabs identified more N. meningitidis asymptomatic carriers than the culture technique. (AU)

FAPESP's process: 11/13290-1 - Prevalence of asymptomatic carriers of Neisseria meningitidis among medical students at the University of São Paulo, Brazil
Grantee:Marta Heloísa Lopes
Support Opportunities: Regular Research Grants