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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples

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Author(s):
Pelizzon, Cristiane ; Carvalho, Carolina da Silva ; Caballero, Susana ; Galetti Junior, Pedro Manoel ; Sanches, Alexandra
Total Authors: 5
Document type: Journal article
Source: CONSERVATION GENETICS RESOURCES; v. 9, n. 1, p. 17-19, MAR 2017.
Web of Science Citations: 2
Abstract

The knowledge of the sex ratio of threatened populations or species is pivotal since sub-optimal sex ratios can affect negatively the population growth and resilience. The vulnerable species, Tapirus terrestris, is rarely studied through traditional field methods and non-invasive genetic analyses have been employed using feces as source of DNA. Here, we evaluated two molecular markers for sex identification of tapirs. Amelogenin gene (AMEL) which is shorter in the Y chromosome (-20pb), failed in the determination of the sex of tapirs, even after the design of specific primers for the species. SRY marker, co-amplified with zinc-finger gene (ZF), had successful produced the sex identity for blood and scat samples. From a total of 45 feaces, 29 had the sex identified after three PCRS (64.4 %). In this way, these molecular markers are reliable tools for sex ratio determination using non-invasive samples of natural populations of this tapirs. (AU)

FAPESP's process: 07/04073-1 - Linking landscape to molecules: genetic analysis of tapir populations (Tapirus terrestris) of the Atlantic rainforest of São Paulo State
Grantee:Alexandra Sanches
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/19377-7 - The effectiveness of the larger atlantic rainforest corridor in the connectivity maintenance of large bodied mammal populations, such as the lowland tapir (Tapirus terrestris)
Grantee:Pedro Manoel Galetti Junior
Support type: Regular Research Grants