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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determining Optimal Fluorescent Agent Concentrations in Dental Adhesive Resins for Imaging the Tooth/Restoration Interface

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Bim Junior, Odair ; Cebim, Marco A. ; Atta, Maria T. ; Machado, Camila M. ; Francisconi-dos-Rios, Luciana F. ; Wang, Linda
Total Authors: 6
Document type: Journal article
Source: Microscopy and Microanalysis; v. 23, n. 1, p. 122-130, FEB 2017.
Web of Science Citations: 2

Fluorescent dyes like Rhodamine B (RB) have been used to identify the spatial distribution of adhesive restorative materials in the tooth/restoration interface. Potential effects of the addition of RB to dental adhesives were addressed in the past, but no further information is available on how to determine suitable concentrations of RB in these bonding agents for imaging in the confocal laser scanning microscope. This study provides systematical strategies for adding RB to viscous dental adhesive resins, focusing on the determination of the lowest range of dye concentrations necessary to achieve an acceptable image of the dentin/adhesive interface. It was demonstrated that optimized images of the resin distribution in dentin can be produced with 0.1-0.02 mg/mL of RB in the (tested) adhesives. Our approaches took into account aspects related to the dye concentration, photophysical parameters in different host media, specimen composition and morphology to develop a rational use of the fluorescent agent with the resin-based materials. Information gained from this work can help optimize labeling methods using dispersions of low-molecular-weight dyes in different monomer blend systems. (AU)

FAPESP's process: 12/13160-3 - Effect of addition of fluorescent agent (Rodhamine B) to no-simplified dentin bonding agents: analisys of physical-mechanical properties
Grantee:Linda Wang
Support Opportunities: Regular Research Grants
FAPESP's process: 11/14971-2 - Evaluation of the effect of adding fluorescent agent (Rhodamine B) in non-simplified adhesive systems.
Grantee:Odair Bim Júnior
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 13/13161-2 - Effect of addition of fluorescein/rhodamine B into non-simplified dentin bonding agents: photophysical and physicochemical approaches
Grantee:Odair Bim Júnior
Support Opportunities: Scholarships in Brazil - Doctorate